Open Access
Table 1.
Primers used in DNA synthesis
| Primer Name | Sequence | Annealing temperature, ° C |
|---|---|---|
| Beta Actin Direct | 5-GGGCATGGGTCAGAAGGATT-3 | 56 |
| Beta actin reverse | 5-GAGGCGTACAGGGATAGCAC-3 | 56 |
| Alpha fetoprotein, direct | 5-TGCAGCCAAAGTGAAGAGGGAAGA-3 | 58 |
| Alpha fetoprotein, reverse | 5-CATAGCGAGCAGCCCAAAGAAGAA-3 | 58 |
| Cytokeratin 18, прямой | 5-ATGGGAGGCATCCAGAACGAGAA-3 | 58 |
| Cytokeratin 18, reverse | 5-GGGCATTGTCCACAGTATTTGCGA-3 | 58 |
| Albumen, direct | 5-TGCTTGAATGTGCTGATGACAGGG-3 | 58 |
| Albumen, reverse | 5-AAGGCAAGTCAGCAGGCATCTCATC-3 | 58 |
| Tryptophan 2,3-Dioxigenase, direct | 5-AGTCAAACC TCCGTGCTT-3 | 58 |
| Tryptophan 2,3-Dioxigenase, reverse | 5-TCGGTGCATCCGAGAAACA-3 | 58 |
| Tyrosinaminotransferase, direct | 5-CTCAATTCTGGACGTGCATG-3 | 52 |
| Tyrosinaminotransferase, reverse | 5-GCTGGTTGGAGAAGATGGCA-3 | 52 |
| Alpha 1 Antitrypsin (Glycoprotein), direct | 5-TCGCTACAGCCTTTGCAATG-3 | 55 |
| Alpha 1 Antitrypsin (Glycoprotein), reverse | 5-TTGAGGGTACGGAGGAGTTCC-3 | 55 |
| Glucuronosyltransferase 1A, direct | 5-TTGCGAACAACACGATACTT-3 | 55 |
| Glucuronosyltransferase 1A, reverse | 5-CAAACTCCACCCAGAACACG-3 | 55 |
| Hepatocyte Nuclear Factor 4 Alpha, direct | 5-GGAACATATGGGAACCAACG-3 | 52 |
| Hepatocyte Nuclear Factor 4 Alpha, reverse | 5-AACTTCCTGCTTGGTGATGG-3 | 52 |
| Cytochrome P450 3A4, direct | 5-TCACCCTGATGTCCAGCAGAAACT-3 | 58 |
| Cytochrome P450 3A4, reverse | 5-TACTTTGGGTCACGGTGAAGAGCA-3 | 58 |
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