Table
Spectral characteristics and concentrations (mg per 100 g DW) of major phenolic compounds in the leaves of F. × ananassa in conditions of field (G) and in vitro culture during multiplication (M) and in vitro rooting (R)
Peak | tr, min | λmax, nm | Compound | G | M | R |
---|---|---|---|---|---|---|
L | 1.8 | 220 | Citric acid | 326.5 ± 18.3a | 210.8 ± 17.2b | 102.6 ± 5.5c |
2 | 2.0 | 220, 250 | Hb | 268.0 ± 11.1a | 278.8 ± 13.4a | 87.8 ± 4.1b |
G | 2.4 | 216, 272 | Gallic acid | 165.3 ± 8.7a | 102.7 ± 3.1b | 74.5 ± 3.7c |
4 | 2.9 | 327 | Hc | 137.8 ± 9.5a | 46.3 ± 3.4c | 54.4 ± 2.4b |
5 | 3.3 | 220, 270 | Hb | 102.6 ± 5.1 | n. d. | n. d. |
6 | 4.0 | 270 | Hb | 101.3 ± 6.4a | 40.3 ± 2.9b | 13.5 ± 0.9c |
7 | 5.5 | 220, 270 | Hb | 180.5 ± 9.3a | 52.7 ± 3.9b | 38.5 ± 2.1 c |
8 | 7.0 | 220, 270 | Hb | 181.2 ± 8.7a | 129.9 ± 8.9b | 60.7 ± 5.6c |
9 | 8.2 | 220, 270 | Hb | 125.4 ± 10.3 | n. d. | n. d. |
C | 8.6 | 218, 240, 324 | Caffeic acid | 56.2 ±3 .8b | 44.0 ± 3.4c | 73.1 ± 2.7a |
11 | 9.4 | 250 | Hb | 66.1 ± 4.3b | 135.3 ± 9.6a | 25.2 ± 1.6c |
12 | 9.7 | 220, 270 | Hb | 86.8 ± 4.2b | 156.3 ± 11.5a | 78.7 ± 2.4c |
pC | 10.8 | 226, 310 | P-coumaric acid | 40.6±1.4 b | 30.5±2.5c | 50.7±1.3a |
14 | 12.3 | 220, 270 | Hb | 17.9 ± 1.1b | 131.9 ± 10.3a | 123.7 ± 7.0a |
F | 13.3 | 218, 236, 320 | Ferulic acid | 24.1 ± 1.2b | 20.3 ± 1.1c | 55.6 ± 2.0a |
16 | 14.1 | 257, 357 | Q glycoside | 1148.7 ± 27.5 | n. d. | n. d. |
17 | 16.3 | 254, 360 | E derivative | 269.3 ± 12.3b | 305.5 ± 16.4a | 250.7 ± 3.1c |
18 | 17.2 | 254, 360 | E derivative | 31.0 ± 1.4c | 174.2 ± 5.9a | 44.9 ± 1.3b |
E | 17.7 | 254, 367 | Ellagic acid | 438.1 ± 20.1a | 312.2 ± 3.4b | 317.4 ± 16.2b |
20 | 19.2 | 265, 356 | K glycoside | 108.8 ± 7.0a | 83.3 ± 4.4b | 97.5 ± 3.8ab |
Total | 3876.2 ± 28.3a | 2255.0 ± 19.7b | 1549.5 ± 57.4c |
tr retention time; Hb hydroxybenzoic acid; Hc hydroxycinnamic acid; n. d. not detected; Q, E, K, and the peak designations correspond with Figure. Means followed by the same letter are not significantly different according to Duncan’s test (P < 0.05).
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