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Table

Spectral characteristics and concentrations (mg per 100 g DW) of major phenolic compounds in the leaves of F. × ananassa in conditions of field (G) and in vitro culture during multiplication (M) and in vitro rooting (R)

Peak tr, min λmax, nm Compound G M R
L 1.8 220 Citric acid 326.5 ± 18.3a 210.8 ± 17.2b 102.6 ± 5.5c
2 2.0 220, 250 Hb 268.0 ± 11.1a 278.8 ± 13.4a 87.8 ± 4.1b
G 2.4 216, 272 Gallic acid 165.3 ± 8.7a 102.7 ± 3.1b 74.5 ± 3.7c
4 2.9 327 Hc 137.8 ± 9.5a 46.3 ± 3.4c 54.4 ± 2.4b
5 3.3 220, 270 Hb 102.6 ± 5.1 n. d. n. d.
6 4.0 270 Hb 101.3 ± 6.4a 40.3 ± 2.9b 13.5 ± 0.9c
7 5.5 220, 270 Hb 180.5 ± 9.3a 52.7 ± 3.9b 38.5 ± 2.1 c
8 7.0 220, 270 Hb 181.2 ± 8.7a 129.9 ± 8.9b 60.7 ± 5.6c
9 8.2 220, 270 Hb 125.4 ± 10.3 n. d. n. d.
C 8.6 218, 240, 324 Caffeic acid 56.2 ±3 .8b 44.0 ± 3.4c 73.1 ± 2.7a
11 9.4 250 Hb 66.1 ± 4.3b 135.3 ± 9.6a 25.2 ± 1.6c
12 9.7 220, 270 Hb 86.8 ± 4.2b 156.3 ± 11.5a 78.7 ± 2.4c
pC 10.8 226, 310 P-coumaric acid 40.6±1.4 b 30.5±2.5c 50.7±1.3a
14 12.3 220, 270 Hb 17.9 ± 1.1b 131.9 ± 10.3a 123.7 ± 7.0a
F 13.3 218, 236, 320 Ferulic acid 24.1 ± 1.2b 20.3 ± 1.1c 55.6 ± 2.0a
16 14.1 257, 357 Q glycoside 1148.7 ± 27.5 n. d. n. d.
17 16.3 254, 360 E derivative 269.3 ± 12.3b 305.5 ± 16.4a 250.7 ± 3.1c
18 17.2 254, 360 E derivative 31.0 ± 1.4c 174.2 ± 5.9a 44.9 ± 1.3b
E 17.7 254, 367 Ellagic acid 438.1 ± 20.1a 312.2 ± 3.4b 317.4 ± 16.2b
20 19.2 265, 356 K glycoside 108.8 ± 7.0a 83.3 ± 4.4b 97.5 ± 3.8ab
Total 3876.2 ± 28.3a 2255.0 ± 19.7b 1549.5 ± 57.4c

tr retention time; Hb hydroxybenzoic acid; Hc hydroxycinnamic acid; n. d. not detected; Q, E, K, and the peak designations correspond with Figure. Means followed by the same letter are not significantly different according to Duncan’s test (P < 0.05).

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