Open Access
Table 3
Effect of growth regulators and media pH on number of germinated spores and number of developed protonemas, prothallia and mature gametophytes of B. multifidum cultured on MS medium media (three replicates of three Petri dishes). Table presents mean values with standard errors, triplicate experiments
| pH | Number of germinated spores (%) | Day germination started | Number of developed protonemas (%) | Number of developed prothalliums (%) | Number of developed mature gametophytes |
|---|---|---|---|---|---|
| NAA 0.1 mg/l | |||||
| 4.8 | 0 | - | 0 | 0 | 0 |
| 5.2 | 0.08±0.01 | 5 | 0.03±0.01 | 0.04±0.01 | 3 |
| 5.6 | 0.84±0.02 | 5 | 0.08±0.01 | 0.07±0.01 | 10 |
| 6.0 | 0.69±0.02 | 6 | 0.05±0.01 | 0.05±0.01 | 6 |
| 6.4 | 0.11±0.01 | 6 | 0.02±0.01 | 0.02±0.01 | 1 |
| IBA 0.1 mg/l | |||||
| 4.8 | - | - | - | - | - |
| 5.2 | 0.15±0.02 | 7 | 0.04±0.01 | 0.03±0.01 | 2 |
| 5.6 | 0.39±0.03 | 7 | 0.02±0.01 | 0.02±0.01 | 4 |
| 6.0 | 0.21±0.01 | 8 | 0.02±0.01 | 0.02±0.01 | 4 |
| 6.4 | 0.09±0.02 | 8 | 0 | 0 | 0 |
| IAA 0.1 mg/l | |||||
| 4.8 | 0 | - | 0 | 0 | 0 |
| 5.2 | 0.10±0.01 | 7 | 0.04±0.01 | 0.03±0.01 | 1 |
| 5.6 | 0.21±0.01 | 8 | 0.05±0.01 | 0.04±0.01 | 4 |
| 6.0 | 0.18±0.02 | 8 | 0.04±0.01 | 0.02±0.01 | 2 |
| 6.4 | 0.07±0.01 | 9 | 0.03±0.01 | 0.03±0.01 | 0 |
| Kinetin 1.0 mg/l | |||||
| 4.8 | 0 | - | 0 | 0 | 0 |
| 5.2 | 0.11±0.02 | 10 | 0.05±0.01 | 0.03±0.01 | 4 |
| 5.6 | 0.60±0.01 | 10 | 0.06±0.02 | 0.04±0.01 | 5 |
| 6.0 | 0.53±0.01 | 11 | 0.04±0.01 | 0.03±0.01 | 0 |
| 6.4 | 0.13±0.01 | 12 | 0.03±0.01 | 0 | 0 |
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