Open Access
Table 2
Primers concentration and fluorochrome label in multiplex PCR.
| Primer | Label | Concentration |
|---|---|---|
| VChr18a-151R | FAM | 0.04 µM |
| VMC2A3 | FAM | |
| VChr1b | NED | |
| FAM116 | VIC | |
| VV6718 | VIC | |
| FAM12 | FAM | 0.05 µM |
| VVC6 | FAM | |
| p3 VvAGL11 | NED | |
| UDV-026 | NED | |
| VVIS58-378R | NED | |
| B003 | PET | |
| VMC8F4.2 | PET | |
| RTAChr16-6b | VIC | |
| VMC7g3 | VIC | |
| VChr18b | PET | 0.07 µM |
| VVIN70 | VIC | |
| VVIB19 | NED | 0.09 µM |
| VV5799 | NED | |
| VVMD32 | VIC | |
| VVIQ67 | FAM | 0.12 µM |
| VRZAG79 | PET | |
| FAM45 | NED | 0.15 µM |
| VMCNG2H2.2 | PET | |
| UDV-095 | VIC | |
| VVIP33 | PET | 0.17 µM |
| VMC4B7-2 | FAM | 0.19 µM |
| VMC4D9-2 | NED | 0.22 µM |
| Polymerase | 1X | |
| DNA | 1-5 ng/µL |
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