Figure 1.

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Sample preparation of Coahuila’s Malbec organic extracts needed to identify PDMS. (1) Left / right: hydroalcoholic solvent elimination. Middle: wine concentrated extract mixed with 20 ml of CCl4 HPLC grade for PDMS solubilization [9] and 42 kHz high-frequency sound waves ultrasonication during 10 minutes at 37oC. (2) Left: after high frequency ultrasonication, mixture is versed into a separatory funnel for phase separation with no further convection. (3) after phase separations, CCl4 extracts are versed in a 100 ml beaker for further concentration. (4) CCl4 excess is eliminated with the aid of a rotary vacuum evaporator until having between 10 and 14 mg of a yellowish oil.
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