Effectiveness of Different Rumen Fluid Fermented Waste Flour in Feed on Glycogen Levels, Protein Retention, and Fat of Vannamei Shrimp

Open Access

Issue		BIO Web Conf. Volume 185, 2025 The International Symposium on Marine and Fisheries (SYMARFISH 2025)


Article Number		08003
Number of page(s)		11
Section		Sustainable Aquaculture and Fisheries
DOI		https://doi.org/10.1051/bioconf/202518508003
Published online		14 August 2025

R.W. Hardy, S.J. Kaushik, (Eds.), Fish nutrition. Academic press. Books [Google Scholar]
X. Li, T. Han, S. Zheng, G. Wu, G. (2021). Nutrition and functions of amino acids in aquatic crustaceans. Amino Acids in Nutrition and Health: Amino Acids in the Nutrition of Companion, Zoo and Farm Animals, 1285. 169–198. (2021). https://link.springer.com/chapter/10.1007/978-3-030-54462-1_8 [Google Scholar]
K. Chakraborty, Recent Advances in Shrimp Nutrition and the Nutritional Significance of Shrimp to Human Health. Shrimp Culture Technology: Farming, Health Management and Quality Assurance, 313–339. (2025). https://link.springer.com/chapter/10.1007/978-981-97-8549-0_18 [Google Scholar]
O. Akintan, K.G. Gebremedhin, D.D. Uyeh, Animal feed formulation Connecting Technologies to Build a Resilient and Sustainable System. Animals, 14, 1497. (2024) https://doi.org/10.3390/ani14101497 [Google Scholar]
Z.A. Kari, S.A.M. Sukri, N.D. Rusli, K. Mat, M.B. Mahmud, N.N.A. Zakaria, N. N. A, W. Wendy, N.K.A. Hamid, M.A. Kabir, N.A.N.A. Arif, S.Z. Abidin, M.K. Zakaria, K.W. Goh, M.I. Khoo, H.V. Doan, A. Tahiluddin, L.S. Wei, Recent advances, challenges, opportunities, product development and sustainability of main agricultural wastes for the aquaculture feed industry–a review. Annals of Animal Science, 23, 25–38. (2023). [CrossRef] [Google Scholar]
A.J. Olaseni, M. Moses, O.A. Mariam, S.S. Oyefunke, Dietary nutrients, antinutritional factors, and valorization of food waste. Food Science and Technology: Trends and Future Prospects, 281. (2020). Proceedings https://www.degruyterbrill.com/document/doi/10.1515/9783111013107-011 [Google Scholar]
H. Yehia, Biochemical Agro-industrial Waste Valorization in Favor of Animal Fodder Application. Bulletin of Pharmaceutical Sciences Assiut University. 48, 606–618 (2025) DOI: 10.21608/bfsa.2025.343927.2375 [Google Scholar]
A.M. Shah, H. Zhang, M. Shahid, H. Ghazal, A.R. Shah, M. Niaz, M. Naz, K. Ghimire, N. Goswami, W. Shi, D. Xia, H. Zhao, The Vital Roles of Agricultural Crop Residues and Agro-Industrial By-Products to Support Sustainable Livestock Productivity in Subtropical Regions. Animals, 15, 1184 (2025) https://doi.org/10.3390/ani15081184 [Google Scholar]
V. Ashokkumar, G. Flora, R. Venkatkarthick, K. SenthilKannan, C. Kuppam, G.M. Stephy, H.K. Wei-Hsin Chen, J. Thomas, C. Ngamcharussrivichai, Advanced Technologies on the Sustainable Approaches for Conversion of Organic Waste to Valuable Bioproducts: Emerging Circular Bioeconomy Perspective. Fuel, 324, (2022). https://doi.org/10.1016/j.fuel.2022.124313 [Google Scholar]
T. Niyigaba, K. Küçükgöz, D. Kołożyn-Krajewska, T. Krolikowski, M. Trząskowska, Advances in Fermentation Technology: A Focus on Health and Safety. Applied Sciences, 15, 3001 (2025) https://doi.org/10.3390/app15063001 [Google Scholar]
Murni, Haryati, S. Aslamyah, H. Sonjaya, The Nutrition Waste Vegetables with Invitro Using Rumen Liquids for Feed. J. Food Nutr. Sci. 6, 58–62 (2018) https://doi.org/10.11648/j.jfns.20180602.13 [Google Scholar]
W.Y. Chuang, L.J. Lin, H.D. Shih, Y.M. Shy, S.C. Chang, T.T. Lee, The potential utilization of high-fiber agricultural by-products as monogastric animal feed and feed additives: A review. Animals, 11, 2098. (2021). https://www.mdpi.com/2076-2615/11/7/2098 [Google Scholar]
P.R.K. Reddy, I. Hyder, Ruminant digestion. Textbook of Veterinary Physiology, 353–366. (2023). https://link.springer.com/chapter/10.1007/978-981-19-9410-4_14 [Google Scholar]
A.E. Kholif, A.K. Patra, Dietary applications of exogenous enzymes to improve nutrient utilization and performance in ruminants. Feed Additives and Supplements for Ruminants, 1–28. (2024). https://link.springer.com/chapter/10.1007/978-981-97-0794-2_1 [Google Scholar]
H. Achmad, M.R. Chaklader, R. Fotedar, M.J. Foysal, From waste to feed: Microbial fermented abalone waste improves the digestibility, gut health, and immunity in marron, Cherax cainii. Fish and Shellfish Immunology, 137, 108748. (2023) https://doi.org/10.1016/j.fsi.2023.108748 [Google Scholar]
Murni, A. Anwar, Aktivitas Enzim Pencernaan Udang Vannamei Yang Diberi Pakan Dengan Penambahan Kadar Tepung Limbah Sayur Terfermentasi Cairan Rumen Kambing Yang Berbeda. Jurnal Ilmiah Ecosystem, 19, 213–217. (2019). https://journal.unibos.ac.id/eco/article/view/894/446 [Google Scholar]
Murni, Haryati, H. Sonjaya, S. Aslamyah, Measuring the substitution of vegetable waste fermented rumen fluid with tofu waste in vannamei shrimp feed. Int. J. Adv. Sci. Eng. Inf. Technol, 9, 2114–2121. (2019) https://ijaseit.insightsociety.org/index.php/ijaseit/article/view/9491 [Google Scholar]
AOAC (Association of Official Analytical Chemists), Official Methods of Analysis, 12th edition. (Washington, D.C: Association of Official Analytical Chemists) p 114. (1990) Books [Google Scholar]
T. Takeuchi, Laboratory work-chemical evaluation of dietry nutrients. Fish nutrition and mariculture, 179–226. (1998) https://scispace.com/papers/laboratory-work-chemical-evaluation-of-dietarynutrients-y41cjpf2qx. [Google Scholar]
G.A. Wedemeyer, W.T. Yasutake, Clinical methods for the assessment of the effects of environmental stress on fish health (No. 89). US Fish and Wildlife Service. (1977). Books [Google Scholar]
Y. Chen, A. Mitra, S. Rahimnejad, S. Chi, V. Kumar, B. Tan, J. Niu, S. Xie, Retrospect of fish meal substitution in Pacific white shrimp (Litopenaeus vannamei) feed: Alternatives, limitations and future prospects. Reviews in Aquaculture, 16, 382–409. (2024). https://doi.org/10.1111/raq.12843 [Google Scholar]
A. Talukdar, A. Dharmendra Deo, N. Prasad Sahu, P. Sardar, M. Aklakur, V. Harikrishna, S. Prakash, N. Shamna, P. Jana, Effects of Different levels of Dietary Protein on the Growth Performance, Nutrient Utilization, Digestive Enzymes and Physiological Status of White Shrimp, Litopenaeus vannamei Juveniles Reared in Inland Saline Water. Aquaculture Nutrition, 27, 77–90. (2021) https://doi.org/10.1111/anu.13166 [Google Scholar]
A.T. Mansour, O.A. Ashry, M. Ashour, A.S. Alsaqufi, K.M. Ramadan, Z.Z. Sharawy, The optimization of dietary protein level and carbon sources on biofloc nutritive values, bacterial abundance, and growth performances of whiteleg shrimp (Litopenaeus vannamei) juveniles. Life, 12, 888. (2022) https://doi.org/10.3390/life12060888 [Google Scholar]
A.E. Kholif, A.K. Patra, Dietary Applications of Exogenous Enzymes to Improve Nutrient Utilization and Performance in Ruminants. Feed Additives and Supplements for Ruminants, 1–28. (2024) Book Chapter. https://link.springer.com/chapter/10.1007/978-981-97-0794-2_1 [Google Scholar]
Kamal, Mahmoud., Aldhalmi, K. Ahmed, El-Hack, M. E. Abd, Elsherbeni, A. I. Youssef, Islam. M. Hussein, Saddam, Bai, Binqiang, Xu, Zhenhua, Hao, Lizhuang, Mahrose, M. Khalid, Taha, Ayman, Cheng, Yanfen, Enhancing the Feed Efficiency of Crop Residues in Ruminants–A Comprehensive Review. Annals of Animal Science, 25, 529–545. (2025) https://www.proquest.com/openview/9315244750725c5f90527b86b793b611/1?pq-origsite=gscholar&cbl=1976406 [Google Scholar]
Y. Chen, A. Mitra, S. Rahimnejad, S. Chi, V. Kumar, B. Tan, J.N.S. Xie, Retrospect of fish meal substitution in Pacific white shrimp (Litopenaeus vannamei) feed: Alternatives, limitations and future prospects. Reviews in Aquaculture, 16, 382–409. (2024) https://doi.org/10.1111/raq.12843 [Google Scholar]
H. Belkhanchi, Y. Ziat, M. Hammi, O. Ifguis, Formulation, optimization of a poultry feed and analysis of spectrometry, biochemical composition and energy facts. South African Journal of Chemical Engineering, 44, 31–41. (2023) https://journals.co.za/doi/abs/10.1016/j.sajce.2023.01.005 [Google Scholar]
X. Deng, K. Chen, D. Jiang, L. Lu, Advancements in synergistic fermentation of probiotics and enzymes for non‐grain feed raw materials. Animal Research and One Health, 3, 31–42 (2025) https://onlinelibrary.wiley.com/doi/full/10.1002 [Google Scholar]
E.M. Alcaide, A.M. García, J.F. Aguilera, A comparative study of nutrient digestibility, kinetics of degradation and passage and rumen fermentation pattern in goats and sheep offered good quality diets. Livestock Production Science, 64, 215–223. (2000) https://doi.org/10.1016/S0301-6226(99)00149-9 [Google Scholar]
S. Kaskous, M.W. Pfaffl, Importance of goat milk for human health and nutrition. Food Nutrition Chemistry. 3, 285. (2025) https://cae.usp-pl.com/index.php/fnc/article/view/ [Google Scholar]
S.S. Sawant, H.Y. Park, E.Y. Sim, H.S. Kim, H.S. Choi, Microbial Fermentation in Food: Impact on Functional Properties and Nutritional Enhancement—A Review of Recent Developments. Fermentation, 11, 15. (2025) https://www.mdpi.com/2311-5637/11/1/15 [Google Scholar]
Murni, A. Anwar, E. Septianingsih, Effect of addition of waste vegetable fermented flour rumen fluid on the quality feed. In IOP Conference Series: Earth and Environmental Science (Vol. 777, No. 1, p. 012028). IOP Publishing. (2021) https://iopscience.iop.org/article/10.1088/1755-1315/777/1/012028 [Google Scholar]
F. Xiao, J. Wang, H. Liu, M. Zhuang, X. Wen, H. Zhao, K. Wu, Effects of dietary protein levels on growth, digestive enzyme activity, antioxidant capacity, and gene expression related to muscle growth and protein synthesis of juvenile greasyback shrimp (Metapenaeus ensis). Animals, 13, 3886. (2023) https://www.mdpi.com/2076-2615/13/24/3886 [Google Scholar]
N.S. Chandel, Carbohydrate metabolism. Cold Spring Harbor perspectives in biology, 13, a040568. (2021) https://cshperspectives.cshlp.org/content/13/1/a040568.short [Google Scholar]
G. Cuzon, C. Rosas, G. Gaxiola, G. Taboada, A. Van Wormhoudt, Utilization of carbohydrates by shrimp. Avances en Nutrición Acuícola. 328–339 (2000) file:///C:/Users/ASUS/Downloads/admin,+23.pdf [Google Scholar]
V.K. Paswan, K. Kumar, A.M. Shehata, Rumen microbiology and microbial degradation of feedstuffs. In Animal Manure: Agricultural and Biotechnological Applications (pp. 45–60). Cham: Springer International Publishing. (2022) https://link.springer.com/chapter/10.1007/978-3-030-97291 [Google Scholar]
Z.K. Liu, Y. Li, C.C. Zhao, Z.J. Liu, L.M. Wang, X.Y. Li, W.F. pellikaan, J.H. Yao, Y.C. Cao, Effects of a combination of fibrolytic and amylolytic enzymes on ruminal enzyme activities, bacterial diversity, blood profile and milk production in dairy cows. animal, 16 (2022). https://www.sciencedirect.com/science/article/pii/S1751731122001483 [Google Scholar]
B.W. Xu, S.S. Li, W.L. Ding, C. Zhang, M.U. Rehman, M.F. Tareen, L. Wang, S.C. Huang, From structure to function: A comprehensive overview of polysaccharide roles and applications. Food Frontiers, 6, 15–39. (2025) https://iadns.onlinelibrary.wiley.com/doi/full/10.1002/fft2.490 [Google Scholar]
C. Py, R. Elizondo‐González, A. Peña‐Rodríguez, Compensatory growth: Fitness cost in farmed fish and crustaceans. Reviews in Aquaculture, 14, 1389–1417. (2022) https://onlinelibrary.wiley.com/doi/abs/10.1111/raq.12656 [Google Scholar]
R.K. Mishra, B. Gamango, A. Jamwal, R.K. Ram, A. Sharma, P. Kumar, Effect of fasting and re-feeding cycles on growth, glucose level, glycogen level, and digestive enzyme activity of Nile tilapia juveniles (Oreochromis niloticus) for cost-effective aquaculture. Aquaculture International, 33, 61. (2025) https://link.springer.com/article/10.1007/s10499-024-01764-1 [Google Scholar]

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