Effect of Bacillus subtilis and Aspergillus niger inoculation on soil physicochemical parameters in agricultural soils from Béni Mellal (Morocco)

Open Access

Issue		BIO Web Conf. Volume 215, 2026 The International Congress on Natural Resources and Sustainable Development (RENA 2025)


Article Number		01010
Number of page(s)		11
Section		Biodiversity and Functioning of Natural Ecosystems
DOI		https://doi.org/10.1051/bioconf/202621501010
Published online		04 February 2026

P.A. Sanchez, Properties and Management of Soils in the Tropics, 2nd ed. (Cambridge University Press, Cambridge, 2019) [Google Scholar]
M. Venkateswarlu, S. Rallapalli, A. Singh, et al., Macro and micronutrient based soil fertility zonation using fuzzy logic and geospatial techniques, Sci. Rep. 15, 26772 (2025). https://doi.org/10.1038/s41598-025-12184-3 [Google Scholar]
S. Elaete, J. Ilukor, R.E. Isoto, et al., Comparing indigenous and scientific (Palin test) soil fertility assessment approaches for better soil health monitoring, management, and farm decision-making in Uganda, Discov. Soil 2, 29 (2025). https://doi.org/10.1007/s44378-025-00058-z [Google Scholar]
S. Fahde, S. Boughribil, L. Ed-daoudy, et al., Effects of physicochemical characteristics of two soils on agro-morphological traits of two chickpea varieties (Cicerarietinum L.), Sci. 7, 45 (2025). https://doi.org/10.3390/sci7020045 [Google Scholar]
Hilali, M. El Baghdadi, A. Barakat, et al., Contribution of GIS techniques and pollution indices in the assessment of metal pollution in agricultural soils irrigated with wastewater: case of the Day River, Beni Mellal (Morocco), Euro-Mediterr. J. Environ. Integr. 5, 52 (2020). https://doi.org/10.1007/s41207-020-00186-8 [Google Scholar]
J.K. Vessey, Plant growth promoting rhizobacteria as biofertilizers, Plant Soil 255, 571–586 (2003). https://doi.org/10.1023/A:1026037216893 [CrossRef] [Google Scholar]
S. Mahapatra, R. Yadav, W. Ramakrishna, Bacillus subtilis impact on plant growth, soil health,and environment: Dr. Jekyll and Mr. Hyde, J. Appl. Microbiol. 132, 3543–3562 (2022). https://doi.org/10.1111/jam.15480 [Google Scholar]
D. Bhardwaj, M.W. Ansari, R.K. Sahoo, N. Tuteja, Biofertilizers function as key player in sustainable agriculture by improving soil fertility, plant tolerance,and crop productivity, Microb. Cell Fact. 13, 66 (2014). https://doi.org/10.1186/1475-2859-13-66 [Google Scholar]
X. Zhang, L. Zhang, J. Liu, et al., Biofertilizers enhance soil fertility and crop yields through microbial community modulation, Agronomy 15, 1572 (2025). https://doi.org/10.3390/agronomy15071572 [Google Scholar]
M. Oueld Lhaj, R. Moussadek, A. Zouahri, et al., Sustainable agriculture through agricultural waste management: a comprehensive review of composting’s impact on soil health in Moroccan agricultural ecosystems, Agriculture 14, 2356 (2024). https://doi.org/10.3390/agriculture14122356 [Google Scholar]
M. Devkota, K.P. Devkota, S. Kumar, Conservation agriculture improves agronomic, economic, and soil fertility indicators for a clay soil in a rainfed Mediterranean climate in Morocco, Agric. Syst. 201, 103470 (2022). https://doi.org/10.1016/j.agsy.2022.103470 [Google Scholar]
K. Jindo, O. El Aroussi, J. de Vente, et al., Effects of local farming practices on soil organic carbon content, enzymatic activities, and microbial community structure in semi-arid soils of Morocco, Front. Soil Sci. 4, 1369971 (2024). https://doi.org/10.3389/fsoil.2024.1369971 [Google Scholar]
M. Bouslim, K. El Mahi, M. Selfati, et al., Microorganisms and bio-sustainability of citrus orchards in Morocco, Int. J. Environ. Stud. 82, 170–181 (2025). https://doi.org/10.1080/00207233.2024.2391724 [Google Scholar]
G. Koskey, S.W. Mburu, R. Awino, et al., Potential use of beneficial microorganisms for soil amelioration, phytopathogen biocontrol, and sustainable crop production in smallholder agroecosystems, Front. Sustain. Food Syst. 5, (2021). https://doi.org/10.3389/fsufs.2021.606308 [Google Scholar]
G.M. Gadd, Geomycology: biogeochemical transformations of rocks, minerals, metals and radionuclides by fungi, bioweathering and bioremediation, Mycol. Res. 111, 3–49 (2007). https://doi.org/10.1016/j.mycres.2006.12.001 [Google Scholar]
N.F. Duarte, C.A. Oliveira Paiva, M.C. Pagano, E.J.A. Correa, Chapter 15 -Phosphate solubilization by microorganisms, in New and Future Developments in Microbial Biotechnology and Bioengineering, H. Singh, A. Vaishnav (Eds.), Elsevier, 257–282 (2022). [Google Scholar]
S.C. Wu, Z.H. Cao, Z.G. Li, et al., Effects of biofertilizer containing N-fixer, P and K solubilizers and AM fungi on maize growth: a greenhouse trial, Geoderma 125, 155–166 (2005). https://doi.org/10.1016/j.geoderma.2004.07.003 [Google Scholar]
Latitude.to, GPS coordinates of Beni Mellal, Morocco. Latitude: 32.3370 Longitude: −6.3565, Latitude.to. [Google Scholar]
Weather and Climate, Afourer, Béni Mellal-Khénifra, MA Climate Zone, Monthly Averages, Historical Weather Data. [Google Scholar]
Supporting report V, Basic study soils, agriculture and irrigation, Japan International Cooperation Agency (undated). [Google Scholar]
J. C. Fernández-Caliani, V. Timón, M.B. Rivera, et al., Experimental and theoretical evidence of zinc structurally bound in vermiculite from naturally metal-enriched soils, Clay Minerals. 48, 3 (2013). https://doi.org/10.1180/claymin.2013.048.3.09 [Google Scholar]
ISO 10390:2021, Sols, biodéchets traités et boues -Détermination du pH, ISO, (Accessed 29 May 2025). [Google Scholar]
ISO 14235:1998, Qualité du sol —Détermination du carbone organique par oxydation sulfochromique, ISO, (Accessed29 May 2025). [Google Scholar]
ISO 11261:1995, Qualité du sol —Dosage de l’azote total —Méthode de Kjeldahl modifiée, ISO, (Accessed 29 May 2025). [Google Scholar]
ISO 11263:1994, Soil quality —Determination of phosphorus —Spectrometric determination of phosphorus soluble in sodium hydrogen carbonate solution, ISO, (Accessed 29 May 2025). [Google Scholar]
ISO 14256-2:2005, Qualité du sol —Dosage des nitrates, des nitrites et de l’ammonium dans des sols bruts par extraction avec une solution de chlorure de potassium —Partie 2: Méthode automatisée avec analyse en flux segmenté, ISO, (Accessed 29 May 2025). [Google Scholar]
AFNOR, NF X31-121 -Qualité des sols -Méthodes chimiques -Détermination du cuivre, du manganèse, du zinc et du fer -Extraction en présence de DTPA, Afnor Éditions, (Accessed 5 Oct 2025). [Google Scholar]
AFNOR, NF X31-122 -Qualité des sols -Extraction et dosage du bore soluble à l’eau bouillante, Afnor Éditions, (Accessed 5 Oct 2025). [Google Scholar]
R Core Team, R: The R Project for Statistical Computing, (Accessed 5 Oct 2025). https://www.r-project.org/ [Google Scholar]
S.S. Shapiro, M.B. Wilk, An analysis of variance test for normality (complete samples), Biometrika 52, 591–611 (1965). https://doi.org/10.1093/biomet/52.3-4.591 [CrossRef] [Google Scholar]
M.B. Wilk, R. Gnanadesikan, Probability plotting methods for the analysis of data, Biometrika 55, 1–17 (1968). https://doi.org/10.1093/biomet/55.1.1 [Google Scholar]
W.J. Conover, Practical Nonparametric Statistics, 3rded., John Wiley & Sons, New York (1999). https://www.scirp.org/reference/referencespapers?referenceid=2919935 [Google Scholar]
W.H. Kruskal, W.A. Wallis, Use of ranks in one-criterion variance analysis, J. Am. Stat. Assoc. 47, 583–621 (1952). https://doi.org/10.1080/01621459.1952.10483441 [Google Scholar]
C. Spearman, The proof and measurement of association between two things, Am. J. Psychol. 15, 72–101 (1904). https://doi.org/10.2307/1412159 [CrossRef] [Google Scholar]
I.T. Jolliffe, Principal Component Analysis, 2nd ed., Springer (2002). https://doi.org/10.1007/b98835 [Google Scholar]
E.E. Idris, H. Bochow, H. Ross, R. Borriss, Use of Bacillus subtilis as biocontrol agent. VI. Phytohormonelike action of culture filtrates prepared from plant growth-promoting Bacillus amyloliquefaciens FZB24, FZB42, FZB45,and Bacillus subtilis FZB37, J. Plant Dis. Prot. 111, 583–597 (2004). [Google Scholar]
M.S. Khan, A. Zaidi, P.A. Wani, Role of phosphate-solubilizing microorganisms in sustainable agriculture: a review, Agron. Sustain. Dev. 27, 29–43 (2007). [CrossRef] [EDP Sciences] [Google Scholar]
B. Nabyl, L. Hanane, E.-K. Houda, et al., Evaluation of the physico-chemical properties of soil and apple leaves (Malus domestica) in Beni Mellal-Khenifra region, Morocco, Adv. Sci. Technol. Eng. Syst. J. 5, 1103–1108 (2020). https://doi.org/10.25046/aj0506134 [Google Scholar]
A.A. Adedayo, O.O. Babalola, Fungi that promote plant growth in the rhizosphere boost crop growth, J. Fungi 9, 239 (2023). https://doi.org/10.3390/jof9020239 [Google Scholar]
M. El Baghdadi, H. Oumeskou, A. Barakat, S. Nadem, J. Rais, Impact of Solid Waste Dump of Beni-Mellal City on Sediments and Soil at Sabeq River, J. Mater. Environ. Sci. 6 (11), 3371–3381 (2015). [Google Scholar]
M. Bonkowski, Protozoa and plant growth: the microbial loop in soil revisited, New Phytol. 162, 617–631 (2004). https://doi.org/10.1111/j.1469-8137.2004.01066.x [Google Scholar]
P.A. Boukdir, U.S.M. Slimane, F.S.T. Béni-Mellal, Caractérisation physico-chimique de la qualité et de la pollution métallique des sols agricoles dans la partie nord-est de la plaine du Tadla, Bassin Oum Er Rbia, Maroc, 2020. [Google Scholar]
Violante, V. Cozzolino, L. Perelomov, et al., Mobility and bioavailability of heavy metals and metalloids in soil environments, J. Soil Sci. Plant Nutr. 10, 268–292 (2010). https://doi.org/10.4067/S0718-95162010000100005 [Google Scholar]
P. Hinsinger, Bioavailability of soil inorganic P in the rhizosphere as affected by root-induced chemical changes: a review, Plant Soil 237, 173–195 (2001). https://doi.org/10.1023/A:1013351617532 [Google Scholar]
T. Samreen, Z.A. Zahir, M. Naveed, M. Asghar, Boron-tolerant phosphorus-solubilizingBacillus spp. MN-54 improved canola growth in alkaline calcareous soils, Int. J. Agric. Biol. 21, 931–940 (2019). https://doi.org/10.17957/IJAB/15.0926 [Google Scholar]
Z. Rengel, Availability of Mn, Zn and Fe in the rhizosphere, J. Soil Sci. Plant Nutr. 15, 397–409 (2015). https://doi.org/10.4067/S0718-95162015005000036 [Google Scholar]
S. Lê, J. Josse, F. Husson, FactoMineR: An R package for multivariate analysis, J. Stat. Softw. 25, 1–18 (2008). https://doi.org/10.18637/jss.v025.i01 [Google Scholar]
C.C. Onoyima, F.G. Okibe, Multivariate analysis of the physico-chemical properties of soils in selected locations of the floodplain of river Kaduna in Niger state, Nigeria, Glob. J. Pure Appl. Sci. 27, 279–288 (2021). https://doi.org/10.4314/gjpas.v27i3.3 [Google Scholar]
Y. Bashan, L.E. de-Bashan, S.R. Prabhu, J.-P. Hernandez, Advances in plant growth-promoting bacterial inoculant technology: formulations and practical perspectives (1998-2013), Plant Soil 378, 1–33 (2014). https://doi.org/10.1007/s11104-013-1956-x [CrossRef] [Google Scholar]
C.-C. Chuang, Y.-L. Kuo, C.-C. Chao, W.-L. Chao, Solubilization of inorganic phosphates and plant growth promotion by Aspergillus niger, Biol. Fertil. Soils 43, 575–584 (2007). https://doi.org/10.1007/s00374-006-0140-3 [Google Scholar]

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