Culture-dependent microbial profiling of Indian smokeless tobacco products

Open Access

Issue		BIO Web Conf. Volume 233, 2026 9^th International Conference on Advances in Biosciences and Biotechnology: Emerging Innovations in Biomedical and Bioengineering Sciences (ICABB 2026)


Article Number		02012
Number of page(s)		16
Section		Environmental Biotechnology and Sustainable Agriculture
DOI		https://doi.org/10.1051/bioconf/202623302012
Published online		23 April 2026

M. Shahid, S. Srivastava, P. Shukla, R. Yadav, M. Sajid, A. Kumar, S. Singh, M. Bharadwaj, Characterization of physiochemical parameters & their effect on microbial content of smokeless tobacco products marketed in north India. Indian J. Med. Res. 158(5-6), 542–551 (2023). https://doi.org/10.4103/iimr.iimr146722 [Google Scholar]
International Agency for Research on Cancer, Smokeless Tobacco and Some Tobacco-related Nitrosamines (IARC Monographs on the Evaluation of Carcinogenic Risks to Humans, Vol. 89). World Health Organization, Lyon (2007). [Google Scholar]
K. Siddiqi, S. Husain, A. Vidyasagaran, A. Readshaw, M. P. Mishu, A. Sheikh, Global burden of disease due to smokeless tobacco consumption in adults: An updated analysis of data from 127 countries. BMC Med. 18,222 (2020). https://doi.org/10.1186/s12916-020-01677-9 [Google Scholar]
D. Hoffmann, S. S. Hecht, Nicotine-derived N-nitrosamines and cancer of the upper digestive and respiratory tracts. Crit. Rev. Toxicol. 15(3), 239–263 (1985). https://pubmed.ncbi.nlm.nih.gov/3882226/ [Google Scholar]
World Health Organization, WHO global report on trends in prevalence of tobacco use 2000-2024 and projections 2025-2030. World Health Organization, Geneva (2025). https://www.who.int/publications/i/item/9789240088283 [Google Scholar]
Tobacco Board, Ministry of Commerce & Industry, Annual Report 2023-24. Government of India (2024). https://tobaccoboard.com/admin/reports/annualreports/ [Google Scholar]
International Institute for Population Sciences, Ministry of Health and Family Welfare, Global Adult Tobacco Survey (GATS 2) India 2016-17. Government of India (2017). [Google Scholar]
B. Sankhla, K. Kachhwaha, S. Y. Hussain, S. Saxena, S. K. Sireesha, A. Bhargava, Genotoxic and carcinogenic effect of gutkha: A fast-growing smokeless tobacco. Addict. Health 10(1), 52–63 (2018). https://doi.org/10.22122/ahj.vl0il,537 [Google Scholar]
A. M. Jacob, J. Jacob, M. M. Mary P. A. K. Shetty, Smoked out or chewed up: Trends in tobacco consumption in India using National Family Health Survey data from 2015 to 2016 and 2019 to 2021. BMJ Open 15(10), e094133 (2025). https://doi.org/10.1136/bmjopen-2024-094133 [Google Scholar]
K. Sathishkumar, M. Chaturvedi, P. Das, S. Stephen, P. Mathur, Cancer incidence estimates for 2022 & projection for 2025: Result from National Cancer Registry Programme, India. Indian J. Med. Res. 156(4-5), 598–607 (2022). https://doi.org/10.4103/ijmr.ijmr182122 [Google Scholar]
T. A. Tali, M. Bashir, B. Ashraf, T. A. Teli, F. Amin, Rising trend of oral cancers in India: A comprehensive review. Int. J. Res. Med. Sci. 13(10), 4547–4550 (2025). https://doi.org/10.18203/2320-6Q12.ijrms20252931 [Google Scholar]
M. Verma, S. V. Singh, R. K. Sahoo, S. Khanna, S. Kumar, NAMS task force report on oral cancer. Ann. Natl. Acad. Med. Sci. (India) 61(3), 272–309 (2025). doi: 10.25259/ANAMS_TFR_12_2024 [Google Scholar]
International Agency for Research on Cancer, Global Cancer Observatory (GLOBOCAN) 2022: India fact sheet. World Health Organization (2024). https://gco.iarc.who.int/today/data/factsheets/populations/356-india-fact-sheets.pdf [Google Scholar]
J. A. Critchley, B. Unai, Health effects associated with smokeless tobacco: A systematic review. Thorax 58(5), 435–443 (2003). https://doi.Org/10.1136/thorax.58.5.435 [Google Scholar]
S. B. Stanfill, G. N. Connolly, R. B. Ennis, N. Ahmed, C. H. Watson, Global surveillance of oral tobacco products: Total nicotine, unionised nicotine and tobacco-specific N- nitrosamines. Tob. Control 20(3), e2 (2011). https://doi.org/10.1136/tc.2010.037465 [Google Scholar]
S. S. Hecht, Biochemistry, biology, and carcinogenicity of tobacco-specific N- nitrosamines. Chem. Res. Toxicol. 11(6), 559–603 (1998). https://doi.org/10.1021/tx980005y [Google Scholar]
S. S. Hecht, D. Hoffmann, Tobacco-specific nitrosamines, an important group of carcinogens in tobacco and tobacco smoke. Carcinogenesis 9(6), 875–884 (1988). https://doi.org/10.1093/carcin/9.6.875 [Google Scholar]
R. Buxton, Nitrate and nitrite reduction test protocols. American Society for Microbiology (2011). https://asm.org/Protocols/Nitrate-and-Nitrite-Reduction-Test-Protocols [Google Scholar]
A. Yadav, P. K. Singh, N. Yadav, P. Lal, J. Ebbert, S. Jain, S. Garg, Smokeless tobacco control in India: Policy review and lessons for high-burden countries. BMJ Glob. Health 5(7),e002367 (2020). https://doi.Org/10.l136/bmigh-2020-002367 [Google Scholar]
M. Sajid, U. Sharma, S. Srivastava, R. K. Yadav, M. Bharadwaj, Microbial community and functions involved in smokeless tobacco product: A metagenomic approach. Appl. Microbiol. Biotechnol. 108,118 (2024). https://doi.org/10.1007/sQ0253-024-13156-9 [Google Scholar]
J. Han, E. K. Lienau, C. Wang, R. Nayak, Bacterial populations associated with smokeless tobacco products. Appl. Environ. Microbiol. 82(20), 6273–6283 (2016). https://doi.org/10.1128/AEM.01612-16 [Google Scholar]
R. E. Tyx, S. B. Stanfill, L. M. Keong, A. J. Rivera, G. A. Satten, C. H. Watson, Characterization of bacterial communities in selected smokeless tobacco products using 16S rDNA analysis. PLoS One 11(1), e0146939 (2016). https://doi.org/10.1371/joumal.pone.0146939 [Google Scholar]
M. Sajid, S. Srivastava, R. K. Yadav, H. Singh, S. Singh, M. Bharadwaj, Composition and ecological functionality of fungal communities associated with smokeless tobacco products mainly consumed in India. Microbiol. Spectr. 10(4), e02273–21 (2022). https://doi.org/10.1128/spectmm.Q2273-21 [Google Scholar]
J. G. Holt, N. R. Krieg, P. H. A. Sneath, J. T. Staley, S. T. Williams, Bergey’s Manual of Determinative Bacteriology, 9th ed. Williams & Wilkins, Baltimore (1994). [Google Scholar]

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