Open Access
| Issue |
BIO Web Conf.
Volume 236, 2026
72nd International Scientific Conference “FOOD SCIENCE, ENGINEERING AND TECHNOLOGY – 2025”
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|---|---|---|
| Article Number | 01004 | |
| Number of page(s) | 8 | |
| Section | Food Science and Technology | |
| DOI | https://doi.org/10.1051/bioconf/202623601004 | |
| Published online | 25 May 2026 | |
- J.P. Tamang, K. Watanabe, W.H. Holzapfel, Diversity of microorganisms in global fermented foods and beverages. Front. Microbiol. 7, 377 (2016). https://doi.org/10.3389/fmicb.2016.00377 [Google Scholar]
- D. Libkind, C.T. Hittinger, E. Valerio, C. Gonçalves, J. Dover, M. Johnston, P. Gonçalves, J.P. Sampaio, Microbe domestication and the identification of the wild genetic stock of lager- brewing yeast. Proc. Natl. Acad. Sci. U.S.A. 108, 14539–14544 (2011). https://doi.org/10.1073/pnas.1105430108 [CrossRef] [PubMed] [Google Scholar]
- W.H. Holzapfel, Appropriate starter culture technologies for small-scale fermentation in developing countries. Int. J. Food Microbiol. 75, 197–212 (2002). https://doi.org/10.1016/S0168-1605(01)00707-3 [Google Scholar]
- N.P. Jolly, C. Varela, I.S. Pretorius, Not your ordinary yeast: non-Saccharomyces yeasts in wine production uncovered. FEMS Yeast Res. 14, 215–237 (2014). https://doi.org/10.1111/1567-1364.12111 [CrossRef] [Google Scholar]
- P. Renault, J. Coulon, G. de Revel, J.C. Barbe, M. Bely, Increase of fruity aroma during mixed T. delbrueckii/S. cerevisiae wine fermentation is linked to specific esters enhancement. Int. J. Food Microbiol. 207, 40–48 (2015). https://doi.org/10.1016/j.ijfoodmicro.2015.04.037 [Google Scholar]
- G. Liti, The Natural History of Model Organisms: The fascinating and secret wild life of the budding yeast Saccharomyces cerevisiae. eLife 4, e05835 (2015). https://doi.org/10.7554/eLife.05835 [Google Scholar]
- M.-A. Lachance, Yeast biodiversity: How many and how much? in Biodiversity and Ecophysiology of Yeasts, The Yeast Handbook, eds. G. Péter, C. Rosa (Springer, Berlin, 2006), pp. 1–9. [Google Scholar]
- P.D. Sniegowski, P.G. Dombrowski, E. Fingerman, Saccharomyces cerevisiae and S. paradoxus coexist in a natural woodland site in North America and display different levels of reproductive isolation from European conspecifics. FEMS Yeast Res. 1, 299–306 (2002). https://doi.org/10.1111/j.1567-1364.2002.tb00048.x [Google Scholar]
- E. Sláviková, R. Vadkertiová, D. Vránová, Yeasts colonizing the leaf surfaces. J. Basic Microbiol. 47, 344–350 (2007). https://doi.org/10.1002/jobm.200710310 [Google Scholar]
- E. Sláviková, R. Vadkertiová, D. Vránová, Yeasts colonizing the leaves of fruit trees. Ann. Microbiol. 59, 419–424 (2009). https://doi.org/10.1007/BF03175125 [Google Scholar]
- S. Malassigné, G. Minard, L. Vallon, E. Martin, C. Valiente Moro, P. Luis, Diversity and functions of yeast communities associated with insects. Microorganisms 9, 1552 (2021). https://doi.org/10.3390/microorganisms9081552 [Google Scholar]
- P. Villarreal, J. Molinet, S. Braun-Galleani, F.A. Cubillos, Non-conventional yeasts as a source of genetic diversity and biotechnological potential. Annu. Rev. Microbiol. 79, 595–614 (2025). https://doi.org/10.1146/annurev-micro-052324-091517 [Google Scholar]
- G.H. Fleet, Yeast interactions and wine flavour. Int. J. Food Microbiol. 86, 11–22 (2003). https://doi.org/10.1016/S0168-1605(03)00245-9 [CrossRef] [Google Scholar]
- R. Jayabalan, R.V. Malbaša, E.S. Lončar, J.S. Vitas, M. Sathishkumar, A review on kombucha tea—Microbiology, composition, fermentation, beneficial effects, toxicity, and tea fungus. Compr. Rev. Food Sci. Food Saf. 13, 538–550 (2014). https://doi.org/10.1111/1541-4337.12073 [Google Scholar]
- G.A. Miguel, S. Carlsen, N. Arneborg, S.M. Saerens, S. Laulund, G.M. Knudsen, Non- Saccharomyces yeasts for beer production: insights into safety aspects and considerations. Int. J. Food Microbiol. 383, 109951 (2022). https://doi.org/10.1016/j.ijfoodmicro.2022.109951 [Google Scholar]
- M. Postaru, A. Tucaliuc, D. Cascaval, A.-I. Galaction, Cellular stress impact on yeast activity in biotechnological processes—a short overview. Microorganisms, 11, 2522 (2023). https://doi.org/10.3390/microorganisms11102522 [Google Scholar]
- W. Albertin, P. Marullo, M. Aigle, C. Dillmann, D. de Vienne, M. Bely, D. Sicard, Population size drives industrial Saccharomyces cerevisiae alcoholic fermentation and is under genetic control. Appl. Environ. Microbiol. 77, 2772–84 (2011). https://doi.org/10.1128/AEM.02547-10 [Google Scholar]
- Y. Kayacan, T. Van Mieghem, F. Delvaux, F.R. Delvaux, R. Willaert, Adaptive evolution of industrial brewer’s yeast strains towards a snowflake phenotype. Fermentation, 6, 20 (2020). https://doi.org/10.3390/fermentation6010020 [Google Scholar]
- N.A. Bokulich, C.W. Bamforth, D.A. Mills, Brewhouse-resident microbiota are responsible for multi-stage fermentation of American coolship ale. PLoS ONE 7, e35507 (2012). https://doi.org/10.1371/journal.pone.0035507 [Google Scholar]
- W. Zhong, T.nChen, H.Yang, E. Li. Isolation and Selection of Non-Saccharomyces Yeasts Being Capable of Degrading Citric acid and Evaluation Its Effect on Kiwifruit Wine Fermentation. Fermentation 6, 25 (2020). https://doi.org/10.3390/fermentation6010025 [Google Scholar]
- R. Alcalá-Jiménez, A. Sánchez-Rodríguez, A. Martínez-Rodríguez, Selection of non- Saccharomyces yeasts from extreme environments for improved wine aroma and flavor. Microorganisms 13, 1260 (2025). https://doi.org/10.3390/microorganisms13061260 [Google Scholar]
- C.P. Boyaci Gunduz, B. Agirman, H. Erten, Identification of yeasts in fermented foods and beverages using MALDI-TOF MS. FEMS Yeast Res. 22, foac056 (2022). https://doi.org/10.1093/femsyr/foac056 [Google Scholar]
- C.P. Kurtzman, R.Q. Mateo, A. Kolecka, B. Theelen, V. Robert, T. Boekhout, Advances in yeast systematics and phylogeny and their use as predictors of biotechnologically important metabolic pathways. FEMS Yeast Res. 15, fov050 (2015). https://doi.org/10.1093/femsyr/fov050 [Google Scholar]
- M. Aydin, S. Kustimur, A. Kalkanci, T. Duran, Identification of medically important yeasts by sequence analysis of the internal transcribed spacer and D1/D2 region of the large ribosomal subunit. Rev. Iberoam. Micol. 36, 129–138 (2019). https://doi.org/10.1016/j.riam.2019.05.002 [Google Scholar]
- A. Baldisseri, D. Santinello, S. Granuzzo, M. Frizzarin, F. De Pascale, G. Sartori, P. Antoniali, S. Campanaro, R. Lopreiato. A Novel PCR-Based Tool to Trace Oenological Saccharomyces cerevisiae Yeast by Monitoring Strain-Specific Nucleotide Polymorphisms. Foods. 14, 13 2379. (2025). https://doi.org/10.3390/foods14132379 [Google Scholar]
- M. de Barros Lopes, S. Rainieri, P.A. Henschke, P. Langridge, AFLP fingerprinting for analysis of yeast genetic variation. Int. J. Syst. Bacteriol. 49, 915–924 (1999). https://doi.org/10.1099/00207713-49-2-915 [Google Scholar]
- C. Longin, F. Julliat, V. Serpaggi, J. Maupeu, G. Bourbon, S. Rousseaux, M. Guilloux-Benatier, H. Alexandre, Evaluation of three Brettanomyces qPCR commercial kits: results from an interlaboratory study. OENO One 50, 223–230 (2016). https://doi.org/10.20870/oeno-one.2016.50.4.1274 [Google Scholar]
- Y. Navarro, M.J. Torija, A. Mas, G. Beltran, Viability-PCR allows monitoring yeast population dynamics in mixed fermentations including viable but non-culturable yeasts. Foods 9, 1373 (2020). https://doi.org/10.3390/foods9101373 [Google Scholar]
- N.A. Bokulich, D.A. Mills, Facility-specific “house” microbiome drives microbial landscapes of artisan cheesemaking plants. Appl. Environ. Microbiol. 79, 5214–5223 (2013). https://doi.org/10.1128/AEM.00934-13 [Google Scholar]
- D.H. Parks, M. Imelfort, C.T. Skennerton, P. Hugenholtz, G.W. Tyson, CheckM: assessing the quality of microbial genomes recovered from isolates, single cells, and metagenomes. Genome Res. 25, 1043–1055 (2015). https://doi.org/10.1101/gr.186072.114 [Google Scholar]
- D.T. Truong, E.A. Franzosa, T.L. Tickle, M. Scholz, G. Weingart, E. Pasolli, A. Tett, C. Huttenhower, N. Segata, MetaPhlAn2 for enhanced metagenomic taxonomic profiling. Nat. Methods 12, 902–903 (2015). https://doi.org/10.1038/nmeth.3589 [Google Scholar]
- R. Binati, N. Ferremi Leali, M. Avesani, E. Salvetti, G.E. Felis, F. Monti, S. Torriani, Application of FTIR microspectroscopy in oenology: shedding light on cell wall composition of Saccharomyces cerevisiae strains. Food Bioprocess Technol. 17, 1596–1609 (2024). https://doi.org/10.1007/s11947-023-03218-7 [Google Scholar]
- M. Potisek, F. Čuš, Monitoring viable yeast populations using flow cytometry in spontaneous and inoculated alcoholic fermentations of white must and red mash. Eur. Food Res. Technol. 251, 2681–2697 (2025). https://doi.org/10.1007/s00217-025-04792-0 [Google Scholar]
- V. Shopska, R. Denkova, V. Lyubenova, G. Kostov, Kinetic characteristics of alcohol fermentation in brewing: state of art and control of the fermentation process. In Fermented Beverages, A.M. Grumezescu, A.M. Holban (Eds.) (Woodhead Publishing, Cambridge 2019), 529–575. https://doi.org/10.1016/B978-0-12-815271-3.00013-0 [Google Scholar]
- P. Zapryanova, Y. Gaytanska, V. Shopska, R. Denkova-Kostova, G. Kostov, Non-Conventional Yeasts for Beer Production—Primary Screening of Strains. Beverages 11, 114 (2025). https://doi.org/10.3390/beverages11040114 [Google Scholar]
- X. Lu, C. Yang, Y. Yang, B. Peng, Analysis of the formation of characteristic aroma compounds by amino acid metabolic pathways during fermentation with Saccharomyces cerevisiae. Molecules 28, 3100 (2023). https://doi.org/10.3390/molecules28073100 [Google Scholar]
- S. Maicas, J.J. Mateo, The life of Saccharomyces and non-Saccharomyces yeasts in drinking wine. Microorganisms 11, 1178 (2023). https://doi.org/10.3390/microorganisms11051178 [Google Scholar]
- D. Shen, X. He, P. Weng, Y. Liu, Z. Wu, A review of yeast: high cell-density culture, molecular mechanisms of stress response and tolerance during fermentation. FEMS Yeast Res. 22, foac050 (2022). https://doi.org/10.1093/femsyr/foac050 [Google Scholar]
- G.-L. Liu, Z. Chi, G.-Y. Wang, Z.-P. Wang, Y. Li, Z.-M. Chi, Yeast killer toxins, molecular mechanisms of their action and their applications. Crit. Rev. Biotechnol. 35, 222–234 (2013). https://doi.org/10.3109/07388551.2013.833582 [Google Scholar]
- D. Marquina, A. Santos, J. Peinado, Biology of killer yeasts. Int. Microbiol. 5, 65–71 (2002). https://doi.org/10.1007/s10123-002-0066-z [Google Scholar]
- P. Zapryanova, Y. Gaytanska, V. Shopska, G. Kostov, Non-conventional yeasts in beer production – a review. BIO Web Conf. 170, 01015 (2025). https://doi.org/10.1051/bioconf/202517001015 [Google Scholar]
- B.E. Wolfe, J.E. Button, M. Santarelli, R.J. Dutton, Cheese rind communities provide tractable systems for in situ and in vitro studies of microbial diversity. Cell 158, 422–433 (2014). https://doi.org/10.1016/j.cell.2014.05.041 [Google Scholar]
- J. Steensels, K.J. Verstrepen, Taming wild yeast: potential of conventional and nonconventional yeasts in industrial fermentations. Annu. Rev. Microbiol. 68, 61–80 (2014). https://doi.org/10.1146/annurev-micro-091213-113025 [Google Scholar]
- N.A. Bokulich, T.S. Collins, C. Masarweh, G. Allen, H. Heymann, S.E. Ebeler, D.A. Mills, Associations among wine grape microbiome, metabolome, and fermentation behavior suggest microbial contribution to regional wine characteristics. mBio 5, e01231–14 (2014). https://doi.org/10.1128/mbio.00631-16 [Google Scholar]
- R. Wei, L. Wang, Y. Ding, L. Zhang, F. Gao, N. Chen, Y. Song, H. Li, H. Wang, Natural and sustainable wine: a review. Crit. Rev. Food Sci. Nutr. 63, 8249–8260 (2023). https://doi.org/10.1080/10408398.2022.2055528 [Google Scholar]
- B. Padilla, J.V. Gil, P. Manzanares, Past and future of non-Saccharomyces yeasts: from spoilage microorganisms to biotechnological tools for improving wine aroma complexity. Front. Microbiol. 7, 411 (2016). https://doi.org/10.3389/fmicb.2016.00411 [Google Scholar]
- S. Ivić, A. Jeromel, B. Kozina, T. Prusina, I. Budić-Leto, A. Boban, V. Vasilj, A.-M. Jagatić Korenika, Sequential fermentation in red wine cv. Babić production: the influence of Torulaspora delbrueckii and Lachancea thermotolerans yeasts on the aromatic and sensory profile. Foods 13, 2000 (2024). https://doi.org/10.3390/foods13132000 [Google Scholar]
- N.A. Bokulich, J.H. Thorngate, P.M. Richardson, D.A. Mills, Microbial biogeography of wine grapes is conditioned by cultivar, vintage, and climate. Proc. Natl. Acad. Sci. U.S.A. 111, E139–E148 (2014). https://doi.org/10.1073/pnas.1317377110 [Google Scholar]
- F. Fejzullahu, Z. Kiss, G. Kun-Farkas, S. Kun, Influence of non-Saccharomyces strains on chemical characteristics and sensory quality of fruit spirit. Foods 10, 1336 (2021). https://doi.org/10.3390/foods10061336 [Google Scholar]
- S.C. Martínez-Estrada, I. Chairez-Hernández, J.A. Narváez-Zapata, J.C. Grijalva-Avila, J.N. Gurrola-Reyes, Yeast population associated with mezcal fermentation. In Integral and Sustainable Use of Agave, eds. A. Gutiérrez Mora et al. (CIATEJ, Zapopan, Mexico, 2019), pp. 95–97. [Google Scholar]
- S. Tireki, A review on packed non-alcoholic beverages: ingredients, production, trends and future opportunities for functional product development. Trends Food Sci. Technol. 112, 442–454 (2021). https://doi.org/10.1016/j.tifs.2021.03.058 [Google Scholar]
- A.J. Marsh, O. O’Sullivan, C. Hill, R.P. Ross, P.D. Cotter, Sequence-based analysis of the bacterial and fungal compositions of multiple kombucha (tea fungus) samples. Food Microbiol. 38, 171–178 (2014). https://doi.org/10.1016/j.fm.2013.09.003 [Google Scholar]
- Y. Wang, Y. Xu, Y. Li, J. Yu, D. Yang, Y. Liu, X. Lin, Flavor formation in water kefir: metabolic roles of microorganisms and their regulatory pathways. Food Rev. Int. (2025), 1–17. https://doi.org/10.1080/87559129.2025.2572780 [Google Scholar]
- A. Shazad, A.Z.A. Tlais, D. Gottardi, P. Filannino, F. Patrignani, R. Lanciotti, M. Gobbetti, R. Di Cagno, Enhancing bioactive profiles of elderberry juice through yeast fermentation: a pathway to low-sugar functional beverages. Curr. Res. Food Sci. 10, 101100 (2025). https://doi.org/10.1016/j.crfs.2025.101100 [Google Scholar]
- N. Singh, S. Gaur, GRAS fungi: a new horizon in safer food product. In Fungi in Sustainable Food Production, eds. X. Dai, M. Sharma, J. Chen (Springer International Publishing, Cham, 2021), pp. 27–37. https://doi.org/10.1007/978-3-030-64406-2_3 [Google Scholar]
- FDA, Generally Recognized as Safe (GRAS). U.S. Food & Drug Administration (2020). Available at: https://www.fda.gov/food/food-ingredients-packaging/generally-recognized-safe-gras [Google Scholar]
- L. Cocolin, V. Alessandria, P. Dolci, R. Gorra, K. Rantsiou, Culture independent methods to assess the diversity and dynamics of microbiota during food fermentation. Int. J. Food Microbiol. 167, 29–43 (2013). https://doi.org/10.1016/j.ijfoodmicro.2013.05.008 [Google Scholar]
- J.-C. Lagier, P. Hugon, S. Khelaifia, P.-E. Fournier, B. La Scola, D. Raoult, The rebirth of culture in microbiology through the example of culturomics to study human gut microbiota. Clin. Microbiol. Rev. 28, 237–264 (2015). https://doi.org/10.1128/CMR.00014-14 [Google Scholar]
- J.E. Aguiar-Cervera, D. Delneri, O. Severn, A high-throughput screening method for the discovery of Saccharomyces and non- Saccharomyces yeasts with potential in the brewing industry. Engineering Biology 5, 72–80 (2021). https://doi.org/10.1049/enb2.12013 [Google Scholar]
- A. Chen, Q. Si, Q. Xu, C. Pan, T. Qu, J. Chen, Evaluation of stress tolerance and fermentation performance in commercial yeast strains for industrial applications. Foods 14, 142 (2025). https://doi.org/10.3390/foods14010142 [Google Scholar]
- G.M. Walker, G.G. Stewart, Saccharomyces cerevisiae in the production of fermented beverages. Beverages 2, 30 (2016). https://doi.org/10.3390/beverages2040030 [Google Scholar]
- L. Alperstein, J.M. Gardner, J.F. Sundstrom, K.M. Sumby, V. Jiranek, Yeast bioprospecting versus synthetic biology—which is better for innovative beverage fermentation? Appl. Microbiol. Biotechnol. 104, 1939–1953 (2020). https://doi.org/10.1007/s00253-020-10364-x [Google Scholar]
- L. Gu, R. Zhang, X. Fan, Y. Wang, K. Ma, J. Jiang, G. Li, H. Wang, F. Fan, X. Zhang, Development of CRISPR/Cas9-based genome editing tools for polyploid yeast Cyberlindnera jadinii and its application in engineering heterologous steroid-producing strains. ACS Synth. Biol. 12, 2947–2960 (2023). https://doi.org/10.1021/acssynbio.3c00278 [Google Scholar]
- S. Li, X. Liu, L. Wang, K. Wang, M. Li, X. Wang, Y. Yuan, T. Yue, R. Cai, Z. Wang, Innovative beverage creation through symbiotic microbial communities inspired by traditional fermented beverages: current status, challenges and future directions. Crit. Rev. Food Sci. Nutr. 64, 10456–10483 (2024). https://doi.org/10.1080/10408398.2023.2225191 [Google Scholar]
- Y. Gonzalez, F. Zea, A. Espinoza, D. Galatro, G. Pilozo, W. Angulo, M.R. Hernandez, J. Urrucsaca, M. Muzzio, M. Rendon-Moran, P. Manzano, Framework for scaling-up extraction processes in nutraceutical beverages: A simulation, techno- economic, and environmental analysis approach. Food Bioprod. Process. 147, 544–553 (2024). https://doi.org/10.1016/j.fbp.2024.08.010 [Google Scholar]
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