BIO Web Conf.
Volume 8, 20172016 International Conference on Medicine Sciences and Bioengineering (ICMSB2016)
|Number of page(s)||7|
|Section||Session I: Medicine|
|Published online||11 January 2017|
Construction and functional analysis of luciferase reporter plasmid containing connexin43 gene promoter
Institute of Biology and Medicine, Wuhan University of Science and Technology, Hubei, China
a Xinghua Liao and Tongcun Zhang (email@example.com) are Corresponding author.
Connexin 43 (Cx43), the principal gap junction protein in vascular smooth muscle cells(VSMCs) resulting in modulation of gap junction. Myocardin is thought to have a key role in Vascular Smooth Muscle Cell (VSMC) development by acting on CArG-dependent genes. In this study, a human Cx43 promoter luciferase reporter construct was generated by PCR amplification of Cx43 promoter. The PCR fragment was digested and cloned into pGL3-Basic vector. The promoter sequence was verified by sequencing. The results showed that luciferase reporter plasmids of Cx43 promoter were successfully constructed. Then the effects of a key transcription factor, which plays important roles in Cx43 levels control, were investigated by luciferase reporter assays in HEK293. The results showed that myocardin can enhance transcriptional activity of Cx43. Our observations provide evidence that Myocardin targets Cx43 expression directly.
© The Authors, published by EDP Sciences, 2017
This is an Open Access article distributed under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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