BIO Web Conf.
Volume 8, 20172016 International Conference on Medicine Sciences and Bioengineering (ICMSB2016)
|Number of page(s)||4|
|Section||Session III: Biomedical Engineering|
|Published online||11 January 2017|
Construction of Yeast One-hybrid Bait Reporter Vector for Screening the Binding Proteins of Cassava MeCWINV1 Promoter
1 Institute of Tropical Biosciences and Biotechnology, Chinese Academy of Tropical Agricultural Sciences / Key Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of Agriculture, Haikou 571101, Hainan, P.R. China
2 College of Agriculture, Hainan University, Haikou 570228, Hainan, P.R. China
a Corresponding author: email@example.com
¶ Jiao Liu, Yuqiang Guo and Ruijun Duan contributed equally to this work
Cellwall invertase (CWIN) hydrolyzes sucrose into glucose and fructose irreversibly, playing key roles in carbohydrate partitioning and plant defence. MeCWINV1 is one of the CWINs in cassava, which contains several light-responsive elements and stress-responsive elements in promoter region. To analyze the regulatory function of MeCWINV1 in the cassava starch accumulation and stress defense response, a 865 bp MeCWINV1 promoter fragment was cloned and inserted into yeast one-hybrid bait vector to construct pCW1-AbAi vector, then was transformed to Y1HGold yeast strains to screen the binding proteins. It might provide a framework for further investigation on the regulation mechanism of MeCWINV1 gene in cassava.
© The Authors, published by EDP Sciences, 2017
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