Study on the identification of Polygonatum kingianum and its adulterants by PCR amplification of specific alleles

¹ Institute of Medical Plant, Yunnan Academy of Agricultural science, Kunming 650223, China
² College of Food Sciences and Technology, Yunnan Agricultural University, Kunming 650092, China
³ Facalty of Sciences and Technology, Kunming University of Sciences and Technology, Kunming 650500, China

^a Corresponding author: pengzhji@126.com

Abstract

Using the allele-specific diagnostic PCR for the rapid and efficient identification of Chinese herbal medicines and their adulterants. In this paper, we analyze the research object of P. kingianum and its adulterants. The total DNA of the samples to be identified was extracted, and the PCR was amplified by using universal primers. After the homology comparison, the specific sites were found out by using BioEdit software, and the specific PCR primers were designed by Premier primer 5.0 software, and the samples were amplified by the primers. The 331bp bands were amplified by P. kingianum of the specific primers, but the results of their adulterants were not amplified. The results show that the method can provide guidance for the allele-specific diagnostic PCR for quick and easy to select P. kingianum, which is not affected by environmental factors, affecting the growth period of the plant, were identified directly from the molecular level of DNA. This method having important application value in accurate introduction and clinical application can provide a model for other identification.