| Issue |
BIO Web Conf.
Volume 16, 2019
“Results and Prospects of Geobotanical Research in Siberia”, dedicated to the 75th anniversary of the laboratory of ecology and geobotany of CSBG SB RAS
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|---|---|---|
| Article Number | 00038 | |
| Number of page(s) | 4 | |
| DOI | https://doi.org/10.1051/bioconf/20191600038 | |
| Published online | 15 October 2019 | |
Various methods for isolating DNA from fruiting bodies on the example of Pleurotus pulmonarius (higher basidiomycetes) of the Novosibirsk Region for barcoding of edible and medicinal mushrooms
Central Siberian Botanical Garden SB RAS, 630090 Zolotodolinskaya st., 101,
Novosibirsk, Russia
* Corresponding author: vlasenkomyces@mail.ru
We conducted experiments to increase the concentration of extracted DNA from the fruiting bodies of this species using various lysis buffers and extraction time. In general, DNA isolation according to the protocol using SDS made it possible to obtain higher concentrations thereof, as in the case of increasing the extraction time from 1 to 24 hours and using additional purification with chloroform.
© The Authors, published by EDP Sciences, 2019
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