Impact of coherent light on interaction of fungi and bacteria cells cultivated in vitro

Michurinsk State Agrarian University, Research Problem Laboratory “Biophotonics”, 393760 Michurinsk, Tambov region, Russia

^* Corresponding author: budagovsky@mail.ru

Abstract

This article considers impact of coherent red quasi-monochromatic light on interaction of colonies of the Pseudomonas syringae bacteria and the Fusarium macroceras fungus in an in vitro culture. A helium-neon laser and a heat source with a system of light filters and aperture diaphragms were used for irradiation. Two light fluxes were obtained with energy parameters close in magnitude, but significantly different in spatio-temporal coherence. Light with a high statistical ordering stimulated growth of both colonies. Irradiation from the same spectral range and intensity, but with low spatial coherence, increased the functional activity of only small bacteria cells. As a result, there was a suppression of larger fungal cells development that were interacting with them. Therefore, it was the statistical (coherent) properties of light that affected the change in the equilibrium of microorganisms in an artificial biocenosis. This approach can be used in practice for increasing the activity of bacteria antagonists of pathogenic fungi and the non-chemical disease protection of plants.