Bacteria as agents of biocontrol of phytopathogens after laser stimulation and of their metabolites’ impact on plants

Michurinsk State Agrarian University, Research Problem Laboratory “Biophotonics”, 393760 Michurinsk, Tambov region, Russia

^* Corresponding author: marinamaslova2009@mail.ru

Abstract

The problem of increasing activity of phytopathogen antagonist bacteria under the influence of laser irradiation is considered in this paper. Studies on Bacillus subtilis and Pseudomonas fluorescens from various plant protection products have shown that use of laser irradiation leads to an increase in the number of cells in bacterial suspensions. This indicator increased to a maximum of two times at effective exposures in comparison with the non-irradiated variants. That was justification for the need to study the nature of the impact of excessive content of metabolites in the substrate synthesized by such a quantity of bacteria from biological products, which is 2 or more times higher than the number of cells in the standard solutions. With a twofold and fourfold increase in the concentration of metabolites of the studied bacteria compared to their standard content in the medium, the photosynthetic activity of cucumber microplants increased by more than 32%. This suggests that laser stimulation of bacteria – agents of biocontrol of diseases does not adversely affect the functional state of plants.