Comparative Studies of In Vitro Regeneration Capacity in Some Breeding Forms of Prunus persica (L.) Batsch

Federal State Funded Institution of Science “The Labor Red Banner Order Nikita Botanical Gardens – National Scientific Center of the RAS”, Plant Developmental Biology, Biotechnology and Biosafety Department, 298648 Yalta, Russian Federation

^* Corresponding author: invitro plant@mail.ru

Abstract

Peach [Prunus persica (L.) Batsch] is one of the most important stone fruit crops in the world. Preservation of valuable genotypes and creation of new breeding forms need the effective methods for plant propagation. Biotechnological method makes it possible to multiply valuable genotypes in vitro and produce high-quality plant material. Plantlets were obtained from hybrid peach embryos in five cross combinations. The induction of morphogenesis and the studies of regenerative capacity were carried out on culture media Murashige, Skoog (MS) and Gamborg, Eveleigh (B5) with vitamins and plant growth regulators. The segments of plantlets with 2-3 internodes were placed on MS and B5 media. Use of B5 medium with 0.75-1.0 mg L^-1 BAP and 0.1 mg L^-1 IBA induced organogenesis in the studied hybrid forms. The microshoots of the hybrid form ‘Summerglo’ × ‘Nikitskiy Podarok’ had a high regeneration capacity. In the forms ‘Persey’ × ‘Nikitskiy Podarok’ and ‘KAT 92-2210’ × ‘Nikitskiy Podarok’ low regeneration capacity was noted. An increase in BAP concentration resulted in formation of hydrated microshoots and non-morphogenic callus. It was determined that to obtain normal peach microshoots, the optimal culture parameters were a temperature of 24 ± 1^oC, 16-hour photoperiod, and 37.5 μM m^-2s^-1 light intensity.