| Issue |
BIO Web Conf.
Volume 24, 2020
International Conferences “Plant Diversity: Status, Trends, Conservation Concept” 2020
|
|
|---|---|---|
| Article Number | 00086 | |
| Number of page(s) | 5 | |
| DOI | https://doi.org/10.1051/bioconf/20202400086 | |
| Published online | 21 September 2020 | |
Hairy roots of pea mutants with a modified morph leaf type
1 Taras Shevchenko Transdnistrian State University, 3300 Tiraspol Transdnistria, Moldova
2 National Ecology and Natural Resources Scientific Research Institute, 3200 Bendery Transdnistria, Moldova
3 Transdniestrian Research Institute of Agriculture, 3300 Tiraspol Transdnistria, Moldova
* Corresponding author: otimina@mail. ru
In the present study, some pea mutants rich in protein with a modified morph leaf type with af and tl genes were genetically transformed using different strains of Agrobacterium rhizogenes A-4, A-48, 15834. Even though a higher total protein content was obtained with the A-48 strain, maximum of the probable transformants were obtained with the A-4 strain but of transient character. The content of the total protein in hairy roots cultures, depending on the composition of the nutrient media and the growth phase of the culture, has been refined.
© The Authors, published by EDP Sciences, 2020
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