| Issue |
BIO Web Conf.
Volume 24, 2020
International Conferences “Plant Diversity: Status, Trends, Conservation Concept” 2020
|
|
|---|---|---|
| Article Number | 00098 | |
| Number of page(s) | 6 | |
| DOI | https://doi.org/10.1051/bioconf/20202400098 | |
| Published online | 21 September 2020 | |
Induction of morphogenesis in the callus culture of Lavandula angustifolia Mill.
1 FSBSI “Research Institute of Agriculture of Crimea”, 295493, Simferopol, Kievskaya str.150, Russia
2 Ufa Institute of biology of UFRC RAS, 450054, Ufa, pr. Oktyabrya, 69, Russia
* Corresponding author: yegorova.na@mail.ru
The influence of the nutrient medium hormonal composition on the induction of calluso- and morphogenesis in Lavandula angustifolia Mill., as well as the content of some endogenous hormones (indolylacetic acid, cytokinins, abscisic acid) in morphogenic and non-morphogenic calli, were studied. The leaf explants were isolated from obtained in vitro plants of the Stepnaya and Vdala cultivars. In primary calli with a frequency of 5.7-11.6%, buds and shoots were formed on different Murashige and Skoog (MS) culture media supplemented with 6-benzylaminopurine, thidiazuron, kinetin, and gibberellic acid. In the callus of the first passage in both cultivars, the induction of morphogenesis was observed with the greatest frequency (39.5-43.2%) on the MS medium with the addition of 1.0 mg/l 6-benzylaminopurine and 1.0 mg/l kinetin. Morphogenic calli of the Vdala cultivar were characterized by a higher content of cytokinins, but a lower content of indolylacetic and abscisic acids compared with non-morphogenic ones.
© The Authors, published by EDP Sciences, 2020
This is an Open Access article distributed under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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