BIO Web Conf.
Volume 24, 2020International Conferences “Plant Diversity: Status, Trends, Conservation Concept” 2020
|Number of page(s)||6|
|Published online||21 September 2020|
Induction of morphogenesis in the callus culture of Lavandula angustifolia Mill.
1 FSBSI “Research Institute of Agriculture of Crimea”, 295493, Simferopol, Kievskaya str.150, Russia
2 Ufa Institute of biology of UFRC RAS, 450054, Ufa, pr. Oktyabrya, 69, Russia
* Corresponding author: firstname.lastname@example.org
The influence of the nutrient medium hormonal composition on the induction of calluso- and morphogenesis in Lavandula angustifolia Mill., as well as the content of some endogenous hormones (indolylacetic acid, cytokinins, abscisic acid) in morphogenic and non-morphogenic calli, were studied. The leaf explants were isolated from obtained in vitro plants of the Stepnaya and Vdala cultivars. In primary calli with a frequency of 5.7-11.6%, buds and shoots were formed on different Murashige and Skoog (MS) culture media supplemented with 6-benzylaminopurine, thidiazuron, kinetin, and gibberellic acid. In the callus of the first passage in both cultivars, the induction of morphogenesis was observed with the greatest frequency (39.5-43.2%) on the MS medium with the addition of 1.0 mg/l 6-benzylaminopurine and 1.0 mg/l kinetin. Morphogenic calli of the Vdala cultivar were characterized by a higher content of cytokinins, but a lower content of indolylacetic and abscisic acids compared with non-morphogenic ones.
© The Authors, published by EDP Sciences, 2020
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