| Issue |
BIO Web Conf.
Volume 25, 2020
International Scientific Online-Conference “Bioengineering in the Organization of Processes Concerning Breeding and Reproduction of Perennial Crops” 2020
|
|
|---|---|---|
| Article Number | 05004 | |
| Number of page(s) | 5 | |
| Section | Getting Healthy Planting Material of Horticultural Crops and Grape Varieties by Means of Bioengineering | |
| DOI | https://doi.org/10.1051/bioconf/20202505004 | |
| Published online | 01 October 2020 | |
Production of diseases - free plant materials of grapevine from phytoplasma in vitro
All-Russian National Research Institute of Viticulture and Winemaking «Magarach», 31 str. Kirova, Yalta, 298600, Republic of Crimea, Russia
* Corresponding author: vik_klim@rambler.ru
Heat treatment was used as a method of improving the health of grape plants from the phytoplasma. The Binder KBWF 240 growth cabinet was used for thermotherapy, in which plants of the Tsitronny Magaracha variety were cultivated in vitro under simulated conditions with a treatment period of 14 days. The results of repeated testing of samples showed the efficacy of treatment of this grape disease: out of 13 samples of the Tsitronny Magaracha variety, only one revealed the latent form of phytoplasma blackening of grapevine wood Bois noir. To improve the health of grape plants obtained in vitro culture, it is advisable to use the developed technological operations with thermotherapy in the growth cabinet, which allows to rid the planting material of the latent form of phytoplasma associated with blackening of grapevine wood Bois noir.
© The Authors, published by EDP Sciences, 2020
This is an Open Access article distributed under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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