BIO Web Conf.
Volume 25, 2020International Scientific Online-Conference “Bioengineering in the Organization of Processes Concerning Breeding and Reproduction of Perennial Crops” 2020
|Number of page(s)||5|
|Section||Getting Healthy Planting Material of Horticultural Crops and Grape Varieties by Means of Bioengineering|
|Published online||01 October 2020|
Micropropagation of some grape varieties in Kazakhstan
Limited Liability Company “Kazakh Research Institute of Fruit & Vegetable Growing”, 238/5 str. Gagarin, Almaty, 050060, Kazakhstan
2 Republican State Enterprise “Institute of Plant Biology and Biotechnology”, 45 str. Timiryazev, Almaty, 050040, Kazakhstan
3 National Joint Stock Company “Kazakh National Agrarian University”, 8 str. Abay, Almaty, 050010, Kazakhstan
* Corresponding author: Saule_5_67@mail.ru
The article shows the improvement of the process of initiation into in vitro the culture and the clonal micropropagation of grape varieties. The optimal culture media for the initiation and cloning of grapes in vitro have been selected. During initiation on Murashige and Skoog, agar medium with ½ or ¾ concentration of macroand micronutrients and hormones (0.5 mg/l BAP and 0.1 mg/l IBA) is optimal. For micropropagation is suitable MS medium modified by some elements of mineral nutrition: 825 mg/l NH4NO3, 166 mg/l CaCl2, 15 mg/l ferrum chelate; best hormonal composition depends on variety: a) 0.5-1 mg/l BAP and 0.1-0.5 mg/l IBA; b) 0.5 mg/l 2-iP and 0.5 mg/l GA3.
© The Authors, published by EDP Sciences, 2020
This is an Open Access article distributed under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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