Influence of mineral and hormonal composition of growing medium on fruit crops and grapes explants development in vitro

¹ Chechen State University, Sheripova Av., 32, Grozny
² Chechen Research Institute of Agriculture, Lenina Av. 1, Grozny

^* Corresponding author: batukaevmalik@mail.ru

Abstract

The influence of the mineral and hormonal composition of the growing medium on the development of explants of fruit crops and grapes in vitro has been studied. As a result of the studies, it has been found that phytohormones have a positive effect on the regeneration of explants in vitro. When using standard compositions of nutrient media not adding auxins, cytokinins and gibberellins, shoot regeneration is reduced, and growth and development are inhibited. The data on the studied media for explants cultivation obtained during the research showed that agarized growing media MS are optimal for microclonal propagation in vitro. Especially this refers to their modifications with a content of 1.0 mg/l 6-BAP at the first planting; a combination of 1.0 mg/l 6-BAP with 1.0 mg/l GK3 during transplantation; 0.5 mg/L IAA at the second transplant. The study of the effect of growth regulators BAP and kinetin combinations on the reproduction factor and the average length of various explants shoots has showed that at the micropropagation stage it is advisable to use BAP and kinetin together at a concentration of 0.5 mg/l each, which ensures the maximum reproduction factor.