Issue |
BIO Web Conf.
Volume 47, 2022
International Scientific and Practical Conference “Innovative Technologies in Agriculture” (ITIA 2022)
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Article Number | 04003 | |
Number of page(s) | 7 | |
Section | Cultures of Isolated Tissues and Organs in Plant Breeding and Reproduction | |
DOI | https://doi.org/10.1051/bioconf/20224704003 | |
Published online | 20 June 2022 |
The effect of growth regulators on the micro-shoots' regeneration of strawberries (Fragaria × ananassa Duch.) in in vitro culture
Russian Research Institute of Fruit Crop Breeding (VNIISPK), 302530, Orel, Russian Federation
* Corresponding author: mazneva@vniispk.ru
The influence of various growth regulators on strawberry varieties' micro-shoots regeneration of Russian and foreign selection Bereginya, Tsaritsa, Urozhaynaya CGL, Frida, Asia, Kimberly, Honeoye, Marmolada has been established. The experiment involved micro-plants regenerated in in vitro culture. At the proliferation stage, 6-BAP, thidiazuron, kinetin, mival, succinic acid were added to the nutrient medium in specified concentrations to select the optimal growth regulator. The maximum response to the induction of additional shoots was obtained in explants cultivated on the control medium of Murashige and Skoog supplemented with 6-BAP. The positive effect of kinetin, thidiazuron, mival, succinic acid on the process of root formation was noted, which can accelerate the production of rooted plants ex vitro.
© The Authors, published by EDP Sciences, 2022
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