Optimization of berry crop genotype regeneration systems at the in vitro crop initiation stage

Russian Research Institute of Fruit Crop Breeding (VNIISPK), Zhilina, Orel district, Orel region, Russian Federation

^* Corresponding author: hromova@vniispk.ru

Abstract

The article presents data obtained during the study of the effectiveness of the initial stage of in vitro introduction of berry crops into culture: black currant (Ribes nigrum L.) and pine strawberry (Fragaria × ananassa Duch.) depending on the period of implementation and sterilizing agents used. The objects of research are varieties of black currant (Azhurnaya, Orlovskaya Serenada, Ocharovanie, Chudnoye Mgnovenye); pine strawberry (Asia, Alba, Bereginya, Darselekt, Kimberly, Klery, Marmolada Onebor, Siria, Honeoye, Florence, Frida, Tsaritsa, Urozhajnaya CGL). Introduction into culture in vitro was carried out in several periods characterized by different physiological state of explants: dormancy release, active growth, and retardation. Cultivation was carried out on Murashige-Skoog medium with the addition of 6-BAP (0.5 mg/l). It is noted that the survival rate of berry crop explants is determined by the physiological state of the source material, due to the period of introduction, and the genotypic characteristics of the varieties. The study of various sterilization modes of the initial plant material showed the effectiveness of such sterilizing agents as hydrogen peroxide (12.0%), merthiolate (0.01%), mercury bichloride (sulema, 0.1%), argentic nitrate (0.2%).