BIO Web Conf.
Volume 47, 2022International Scientific and Practical Conference “Innovative Technologies in Agriculture” (ITIA 2022)
|Number of page(s)||9|
|Section||Cultures of Isolated Tissues and Organs in Plant Breeding and Reproduction|
|Published online||20 June 2022|
Optimization of berry crop genotype regeneration systems at the in vitro crop initiation stage
Russian Research Institute of Fruit Crop Breeding (VNIISPK), Zhilina, Orel district, Orel region, Russian Federation
* Corresponding author: email@example.com
The article presents data obtained during the study of the effectiveness of the initial stage of in vitro introduction of berry crops into culture: black currant (Ribes nigrum L.) and pine strawberry (Fragaria × ananassa Duch.) depending on the period of implementation and sterilizing agents used. The objects of research are varieties of black currant (Azhurnaya, Orlovskaya Serenada, Ocharovanie, Chudnoye Mgnovenye); pine strawberry (Asia, Alba, Bereginya, Darselekt, Kimberly, Klery, Marmolada Onebor, Siria, Honeoye, Florence, Frida, Tsaritsa, Urozhajnaya CGL). Introduction into culture in vitro was carried out in several periods characterized by different physiological state of explants: dormancy release, active growth, and retardation. Cultivation was carried out on Murashige-Skoog medium with the addition of 6-BAP (0.5 mg/l). It is noted that the survival rate of berry crop explants is determined by the physiological state of the source material, due to the period of introduction, and the genotypic characteristics of the varieties. The study of various sterilization modes of the initial plant material showed the effectiveness of such sterilizing agents as hydrogen peroxide (12.0%), merthiolate (0.01%), mercury bichloride (sulema, 0.1%), argentic nitrate (0.2%).
© The Authors, published by EDP Sciences, 2022
This is an Open Access article distributed under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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