BIO Web Conf.
Volume 47, 2022International Scientific and Practical Conference “Innovative Technologies in Agriculture” (ITIA 2022)
|Number of page(s)||6|
|Section||Cultures of Isolated Tissues and Organs in Plant Breeding and Reproduction|
|Published online||20 June 2022|
Some clonal micropropagation features of Lonicera caerulea L. cultivars in vitro
Tsitsin Main Botanical Garden of Russian Academy of Sciences, 127276 Botanicheskaya street 4, Moscow, Russian Federation
* Corresponding author: Irosvet96@mail.ru
Lonicera caerulea L. is one of the early temperate berry crops. Honeysuckle is a deciduous shrub up to 2.5 m high with edible blue fruits rich in vitamins and polyphenols. The work is devoted to the improvement of in vitro propagation method of some Lonicera caerulea cultivars. The differences in regeneration and formation of axillary shoots were revealed depending on the mineral medium composition. The optimal mineral composition of the nutrient medium for clonal micropropagation of the studied genotypes was determined. Cultivation on of Quoirin and Lepoivre medium contributed to the maximum reproduction coefficient (14.3) and height of microshoots (5.1 cm). The optimal concentration of auxin at the rooting stage has been established. The highest percentage of rooted microshoots (91%) was noted on a nutrient medium with 0.3 mg/l indoleacetic acid.
© The Authors, published by EDP Sciences, 2022
This is an Open Access article distributed under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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