BIO Web Conf.
Volume 36, 2021International Scientific and Practical Conference “Fundamental Scientific Research and Their Applied Aspects in Biotechnology and Agriculture” (FSRAABA 2021)
|Number of page(s)||6|
|Section||Soil Resources and Management of Soil Fertility Parameters|
|Published online||01 October 2021|
Influence of different concentrations of 6-benzylaminopurine and thidiazuron on the proliferative activity of apple varieties in in vitro culture
Russian Research Institute of Fruit Crop Breeding (VNIISPK), Zhilina, Orel district, Orel region, Russian Federation
* Corresponding author: email@example.com
An essential role in the process of clonal micropropagation is played by cytokinins, with the help of which apical dominance is removed and the formation of additional shoots and buds is activated. The article presents the study results of microshoots’ proliferative activity of apple varieties on media with 6-benzylaminopurine at a concentration of 1.0 mg/l, 2.0 mg/l and thidiazuron at a concentration of 0.1 mg/l, 0.2 mg/l and 0.3 mg/l. The objects of research were apple varieties of the VNIISPK selection - Bolotovskoe, Imrus, Veteran, Kandil orlovsky, Girlyanda, Priokskoe. The aim of the research was to study the effect of substances with cytokinin activity on the morphogenesis of apple varieties. As a result of the research, it was revealed that both cytokinins and varietal characteristics influence the multiplication factor. The highest multiplication factor was observed when using 6-BAP. Thidiazuron reduced the proliferation rate. When using 6-BAP, Bolotovskoe, Girlyanda and Priokskoe varieties had the highest multiplication factor among the varieties. When using TDZ - varieties Bolotovskoe and Kandil Orlovsky.
© The Authors, published by EDP Sciences, 2021
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