| Issue |
BIO Web Conf.
Volume 47, 2022
International Scientific and Practical Conference “Innovative Technologies in Agriculture” (ITIA 2022)
|
|
|---|---|---|
| Article Number | 04002 | |
| Number of page(s) | 8 | |
| Section | Cultures of Isolated Tissues and Organs in Plant Breeding and Reproduction | |
| DOI | https://doi.org/10.1051/bioconf/20224704002 | |
| Published online | 20 June 2022 | |
Effect of colchicine on meristematic tissues of diploid apple varieties in in vitro culture
Russian Research Institute of Fruit Crop Breeding (VNIISPK), Orel region, Russia
* Corresponding author: tashmatova@vniispk.ru
For apple tree selection, it is of great importance to transfer diploid varieties and hybrids with already known valuable properties to the polyploid level. Tetraploid apple varieties are of interest for breeding as donors of non-reduced gametes in the production of triploids. The purpose of these studies was to study the effect of colchicine on the viability of explants in in vitro culture and their further growth and development. The objects of the study were diploid apple varieties. Polyploidization was carried out by cultivation on an agarized nutrient medium containing colchicine in concentrations of 0.01% and 0.02% and by drip method. The study showed that prolonged exposure to colchicine causes the death of explants. The genotypic reaction of the varieties to the processing methods, the concentration of amitotic, and the duration of its exposure were noted. Explants of the Imrus variety turned out to be more sensitive to the action of colchicine.
© The Authors, published by EDP Sciences, 2022
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