Micropropagation of Asparagus officinalis L. through callus development

The University of Danang-University of Science and Education, ul. Ton Duc Thang, 459, Da Nang, Vietnam
Ming Chi University of Technology, 84 Gungjuan Rd., Taishan Dist., New Taipei City 243303, Taiwan
Russian State University - Moscow Agricultural Academy named after K. A. Timiryazev, 49 Timiryazevskaya St., Moscow 127550, Russia

^* Corresponding author: a.mironov@rgau-msha.ru

Abstract

In order to develop an in vitro propagation process for production and conservation, in this study, we investigated the influence of disinfectants and plants growth regulators (PGR) on the infection rate of explants, the ability of callus formation, and the regeneration of shoot and root from Asparagus officinalis L. trunk segments. We obtained the best results when disinfecting trunk segments explants combined with alcohol 70° in 5 minutes and NaClO in 5 minutes with the lowest contamination rate of 3.7%. Callus was formed well in MS medium supplemented with 3% sucrose; 0.7% agar, pH= 5.8; 1.0 mg/l BAP and combined with 0.5 mg/l NAA, 1.5 mg/l BAP and 0.75 mg/l NAA. The highest rate of shoot formation was 87.2% when the callus continued to grow in the environment supplemented with 2.0 mg/l BAP and 1.0 mg/l NAA. Roots were formed after 4 weeks of shoot cultivation in MS medium supplemented with 0.5 mg/l NAA with the highest rooting rate of 74.59%.