| Issue |
BIO Web Conf.
Volume 30, 2021
II International Symposium “Innovations in Life Sciences” (ILS 2020)
|
|
|---|---|---|
| Article Number | 05004 | |
| Number of page(s) | 5 | |
| Section | Fundamental and Applied Aspects of Biotechnology and Microbiology | |
| DOI | https://doi.org/10.1051/bioconf/20213005004 | |
| Published online | 22 April 2021 | |
The study of the consequences of preserving berry crops in vitro on the processes of subsequent clonal micropropagation
Federal State Budgetary Scientific Institution «Federal Scientific Agroengineering Center VIM», All-Russian Horticultural Institute for Breeding, Agrotechnology and Nursery Moscow, Russia
* Corresponding author: knyazewa.inna@yandex.ru
As a result of studies, it was found that collection samples of berry crops in vitro can be maintained in viable condition on specially selected nutrient media for 12 months. The effect of mannitol concentration (0.45, 0.75 and 1.05%; sucrose control -3%) on the regenerative potential of microprobe of the studied berry crop varieties was estimated. When studying the effect of the reproduction cycle on the morphogenesis of meristemic cultures, it was found that the dynamics of changes in shoot length, number of leaves and shoots depended on the concentration of the active substance. In general, the majority of berry crops that were viable after storage of explants retained the ability to grow again, in many cases reaching or exceeding the control level.
© The Authors, published by EDP Sciences, 2021
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