BIO Web Conf.
Volume 30, 2021II International Symposium “Innovations in Life Sciences” (ILS 2020)
|Number of page(s)||6|
|Section||Biosafety as the Basis of Pharmaceutical Production and Veterinary Medicine|
|Published online||22 April 2021|
Development of approaches to the diagnosis of cattle leukemia in the system of antiepizootic measures in the Belgorod Region
Belgorod State Agrarian University named after V. Ya. Gorin, vil. Mayskiy, Russia
2 Russian Academy of Sciences, Moscow, Russia
3 Veterinary Department of the Belgorod region, Belgorod, Russia
4 Ural Federal Agrarian Research Center of the Ural Branch of the Russian Academy of Sciences, Yekaterinburg, Russia
* Corresponding author: firstname.lastname@example.org
The aim of the research is the use of serological and molecular genetic methods for detecting virus-infected cattle leukemia, as well as determining the significance of PCR in identifying BLV infected calves in the system of antiepizootic health measures. The developed technique for early diagnosis of leukemia in cattle made it possible to accelerate the process of recovery of disadvantaged farms in the Belgorod region by increasing the frequency of studies from 6 months to 2-3 months and an increase in the sensitivity of the agar-gel immunodiffusion test. This, in turn, leads to an increase in the sensitivity of the agar-gel immunodiffusion test and makes it possible to detect, on average, from 8.8% to 20.25% more animals infected with the leukemia virus compared to the standard reaction of the agar-gel immunodiffusion test. The additional use of molecular genetic tests for the detection of proviral DNA of the leukemia virus makes it possible to identify at the early stages of the development of the leukemia process, in calves from 15 days of age, the genomic material of bovine leukemia virus, which will also allow in a shorter time to carry out a qualitative improvement of young cattle in dysfunctional farms.
© The Authors, published by EDP Sciences, 2021
This is an Open Access article distributed under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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