Identification of phenolic constituents in Lonicera caerulea L. by HPLC with diode array detection electrospray ionisation tandem mass spectrometry

¹ N.I. Vavilov All-Russian Institute of Plant Genetic Resources, B. Morskaya 42-44, 190000, Saint-Petersburg, Russian Federation
² Far Eastern Federal University, Sukhanova 8, 690950, Vladivostok, Russian Federation
³ Siberian Federal Scientific Centre of Agrobiotechnology, 633501, Centralnaya, Presidium, Krasnoobsk, Russian Federation
⁴ Pacific Geographical Institute, Far Eastern Branch of the Russian Academy of Sciences, Radio 7, 690041, Vladivostok, Russian Federation

^* Corresponding author: razgonova.mp@dvfu.ru (M.R.)

Abstract

The purpose of this work was a comparative metabolomic study of extracts of Blueberried honeysuckle Lonicera caerulea L.: №1043-11 (St. Petersburg); №1043-08 (St. Petersburg) №863; (Japan); №860 (Wild Lonicera from Amur river) from the collection of N.I. Vavilov All-Russian Institute of Plant Genetic Resources. To identify target analytes in extracts HPLC was used in combination with a BRUKER DALTONIKS ion trap. The results showed the presence of 82 target analytes corresponding to family Caprifoliaceae. In addition to the reported metabolites, a number of metabolites were newly annotated in Lonicera caerulea L. There were flavonols: Dihydrokaempferol, Rhamnetin I, Rhamnetin II, Taxifolin-3O-glucoside, Mearnsetin-hexoside, Horridin; flavones: Chrysoeriol, Apigenin-O-pentoside, Chrysoeriol-7-Oglucoside; flavanone Naringenin; flavan-3-ols: Catechin, Epicatechin, Biochanin A-7-O-glucoside; essential amino acids: L-Pyroglutamic acid, Tyrosine; polypeptide 5-Oxo-L-propyl-L-isoleucine; sterols: Ergosterol, Fucosterol, Beta-Sitosterin; triterpenoids: Betunolic acid, Oleanoic acid; anabolic steroid Vebonol, indole sesquiterpene alkaloid Sespendole; iridoids: Monotropein, p-Coumaroyl monotropein, p-Coumaroyl monotropein hexoside; Myristoleic acid, etc.