Issue |
BIO Web Conf.
Volume 33, 2021
The 1st International Conference of Advanced Veterinary Science and Technologies for Sustainable Development (ICAVESS 2021)
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Article Number | 06009 | |
Number of page(s) | 7 | |
Section | Veterinary Medicine and Science | |
DOI | https://doi.org/10.1051/bioconf/20213306009 | |
Published online | 23 August 2021 |
Isolation, Identification, and Antimicrobial Susceptibility Test of Bacteria from Vulva Swab of African Pygmy Hedgehog (Atelerix albiventris) and Sunda Porcupine (Hystrix javanica)
1
Student in Faculty of Veterinary Medicine, Universitas Gadjah Mada, Indonesia
2
Department of Biology, Faculty of Biotechnology, Universitas Kristen Duta Wacana, Indonesia
3
Department of Reproduction and Obstetrics, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Indonesia
4
Department of Anatomy, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Indonesia
5
Department of Microbiology, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Indonesia
* Corresponding author: wahyun_aeth@ugm.ac.id
Atelerix albiventris and Hystrix javanica are currently traded as pets or consumed in Indonesia, but there has been no research about bacteria from the vulva swab before. This research aims to isolate and identify bacteria from the vulva swabs of Atelerix albiventris and Hystrix javanica, and identify their antibiotic susceptibility. Samples were isolated by blood agar plates and selective media and identified by biochemical tests. Kirby Bauer’s disk diffusion method was used for the antimicrobial susceptibility test. The result showed that from Atelerix albiventris was isolated and identified Escherichia coli and Proteus mirabilis, while from Hystrix javanica was isolated and identified Escherichia coli. The identified Escherichia coli was sensitive to Amikacin, Amoxycillin, Ampicillin, Enrofloxacin, Fosfomycin, Chloramphenicol, Tetracycline, Trimethoprim, and Kanamycin; intermediate to Streptomycin; and resistant to Erythromycin and Penicillin G. The identified Proteus mirabilis was sensitive to Amikacin, Amoxycillin, Kanamycin, Enrofloxacin, and Fosfomycin; and resistant to Erythromycin, Penicillin G, Streptomycin, Ampicillin, Chloramphenicol, Tetracycline, and Trimethoprim. This research concludes that Escherichia coli and Proteus mirabilis were isolated from vulva swab of Atelerix albiventris and Hystrix javanica, Escherichia coli was sensitive to eight antibiotics, while Proteus mirabilis was sensitive to five antibiotics.
© The Authors, published by EDP Sciences, 2021
This is an Open Access article distributed under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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