The mice’s hematological effect of given the Staphylococcus aureus and Persea americana

Department of Clinical Pathology, Faculty of Veterinary Medicine, Universitas Gadjah Mada Yogyakarta, Yogyakarta Special Region, Indonesia

^* Corresponding author: jchrist@ugm.ac.id

Abstract

Staphylococcus aureus is often the cause of various diseases and food poisoning in animals and humans. Avocado seeds (Persea americana, Mill) contain important compounds that have antimicrobial activity. This study aimed to observe the hematological dynamics of mice (Mus musculus domesticus) infected with S. aureus and induced by P. americana extract. BALB/c male mice aged 12 weeks were divided into 6 groups of 5 in each group, named K-1, K-2, K-3, K-4, K-5, and K-6. All groups were infected with S. aureus 1x10⁸ CFU cell/mL intraperitoneally (IP) on day 0. After the blood sample was taken, then K-1 was given a single dose of tetracyclin 130 mg/kg BW, K-2 was given 130 mg/kb BW oferythromycin, K-3 was given aquabidest (placebo), K-4 was given P. americana extract 300 mg/kg BW, K-5 at a dose 600 mg/kg BW and K-6 at a dose 1200 mg/kg BW. The treatment was given for 7 consecutive days, then a second blood sample was taken and analyzed with the VetScan-HM5 analyzer and interpreted. It was concluded that S. aureus infection caused leukopenia and was potentially followed by lymphopenia, neutrophilia, and monocytosis. The occurrence of microcytosis can lead to anemia, which can be categorized as microcytic hypochromic or normocytic normochromic. Administration of P. americana extract at a dose 300 mg/kg BW (K-4) for 7 days after S. aureus infection was found to be effective in improving hematological values in mice back to normal.