BIO Web Conf.
Volume 36, 2021International Scientific and Practical Conference “Fundamental Scientific Research and Their Applied Aspects in Biotechnology and Agriculture” (FSRAABA 2021)
|Number of page(s)||7|
|Section||Biotechnology in the Agro-Industrial Complex|
|Published online||01 October 2021|
Evaluation of the effectiveness of potato improvement in in vitro culture on optimized nutrient media
Federal State Budgetary Educational Institution of Higher Education “Orel State Agrarian University named after N.V. Parakhin”, 69 Generala Rodina Str., city of Orel, 302019, Russia
* Corresponding author: SolohinaIrina@yandex.ru
Studies have been conducted on the use of an optimized Murashige-Scoog nutrient medium to obtain healthy seeds of promising potato varieties. It was found that the use of agarized modified Murashige-Scoog nutrient medium with the addition of exometabolites of fungi of the genus Trichoderma lixii 1.5 mg/l with antimicrobial properties and the antiviral drug “Cycloferon” in a concentration of 0.01% contributes to an increase in the number of virus-free potato regenerants when the method of apical meristems and in vitro improvement is used together. The effectiveness of using the modified medium is confirmed by the following criteria: active growth of test tube potato micro-plants, which is expressed in optimal morphometric indicators on the example of the potato variety “Rosara”; the size of the isolated meristems is 10 mm, which contributes to the output of healthy plants; the survival rate of regenerating plants. It is shown that when using the modified medium, an average of 35-50% of regenerating plants were obtained, depending on the potato variety, free from M, S viruses.
© The Authors, published by EDP Sciences, 2021
This is an Open Access article distributed under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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