| Issue |
BIO Web Conf.
Volume 38, 2021
Northern Asia Plant Diversity: Current Trends in Research and Conservation 2021
|
|
|---|---|---|
| Article Number | 00054 | |
| Number of page(s) | 5 | |
| DOI | https://doi.org/10.1051/bioconf/20213800054 | |
| Published online | 28 October 2021 | |
Analysis of intraspecific polymorphism of Nitraria sibirica Pall. using the ISSR technique
Central Siberian Botanical Garden SB RAS, 630090 Novosibirsk, Russia
* Corresponding author: skhozyaykina@gmail.com
The analysis of DNA polymorphism of Nitraria sibirica Pall. was carried out at 13 natural populations of the Republic of Altai and Altai Territory using the ISSR technique. Seven effective ISSR primers have been identified to analyze DNA polymorphism in N. sibirica. 99 DNA fragments were yielded at DNA amplification with these primers, 66 of them were polymorphic. The genetic distance Nei (D) between the studied populations of N. sibirica averaged 0.32, at mean 0.09 - within populations. An identification ISSR marker has been revealed, which can be used to study the genetic variability of the genus Nitraria L. (Nitrariaceae) species.
© The Authors, published by EDP Sciences, 2021
This is an Open Access article distributed under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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