| Issue |
BIO Web Conf.
Volume 38, 2021
Northern Asia Plant Diversity: Current Trends in Research and Conservation 2021
|
|
|---|---|---|
| Article Number | 00082 | |
| Number of page(s) | 4 | |
| DOI | https://doi.org/10.1051/bioconf/20213800082 | |
| Published online | 28 October 2021 | |
ISSR primer screening for analysis of genetic diversity among Scutellaria tuvensis (Lamiaceae) populations
Central Siberian Botanical Garden SB RAS, 630090 Novosibirsk, Russia
* Corresponding author: dsmuraseva@csbg.nsc.ru
Using the Diamond DNA kit, high quality nuclear DNA was isolated from dry leaves of the endemic species Scutellaria tuvensis. The selection of primers for ISSR analysis of genetic polymorphism of natural populations is described. During the experiment, 22 primers were tested, their effectiveness was assessed on a point scale. When assessing the primers, the number of reproducible amplified DNA fragments, the clarity and brightness of the obtained fragments, and only distinctive bands were taken into account. As a result, 10 ISSR markers were selected that are the most informative for assessing the population diversity of the species.
© The Authors, published by EDP Sciences, 2021
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