| Issue |
BIO Web Conf.
Volume 39, 2021
International Scientific and Practical Conference “Modern Trends in Science, Innovative Technologies in Vineyards and Wine Making” (MTSITVW2021)
|
|
|---|---|---|
| Article Number | 02004 | |
| Number of page(s) | 12 | |
| Section | Genetic Resources, Genetics, Genomics, Bioengineering | |
| DOI | https://doi.org/10.1051/bioconf/20213902004 | |
| Published online | 26 November 2021 | |
Simultaneous determination of polyphenol content Vitis amurensis Rupr. by tandem mass spectrometry
1 N.I. Vavilov All-Russian Institute of Plant Genetic Resources, B. Morskaya 42-44, 190000, Saint-Petersburg, Russian Federation
2 Far Eastern Federal University, Sukhanova 8, 690950, Vladivostok, Russian Federation
3 Pacific Geographical Institute, Far Eastern Branch of the Russian Academy of Sciences, Radio 7, 690041, Vladivostok, Russian Federation
4 Siberian Federal Scientific Centre of Agrobiotechnology, 633501, Centralnaya, Presidium, Krasnoobsk
* Corresponding author: m.razgonova@vir.nw.ru
Vitis amurensis Ruprecht contains a large number of polyphenolic compounds which are biologically active components. For the most efficient and safe extraction supercritical carbon dioxide was used. In this work, for the first time, a comparative metabolomic study of biologically active substances of wild grapes collected from five different places of the Primorsky and Khabarovsk territories is carried out. To identify target analytes in ethanol extracts of grape berries, high performance liquid chromatography (HPLC) was used in combination with an amaZon SL ion trap (manufactured by BRUKER DALTONIKS, Germany) equipped with an ESI electrospray ionization source in negative and positive ion modes. The mass spectrometer was used in the scan range m / z 100 - 1.700 for MS and MS / MS. Used fragmentation of the 4th order. Primary mass spectrometric results showed the presence of 94 biologically active compounds corresponding to the species V. amurensis, moreover, salvianolic acids F, D and G, oleanoic, ursolic, myristoleic acids, berbericinin, mearnsetin, esculin, nevadensin, stigmasterol, fucosterol, phlorizin, L-tryptophan identified for the first time in V. amurensis.
© The Authors, published by EDP Sciences, 2021
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