| Issue |
BIO Web Conf.
Volume 39, 2021
International Scientific and Practical Conference “Modern Trends in Science, Innovative Technologies in Vineyards and Wine Making” (MTSITVW2021)
|
|
|---|---|---|
| Article Number | 03002 | |
| Number of page(s) | 5 | |
| Section | Agro-Technological Systems of Cultivation | |
| DOI | https://doi.org/10.1051/bioconf/20213903002 | |
| Published online | 26 November 2021 | |
Development of in vitro culture establishment conditions and micropropagation of grapevine rootstock cultivar ‘Ruggeri-140’
1 Scientific Center of Agrobiotechnology, branch of Armenian National Agrarian University, 1101, Etchmiadzin, Isi-Le-Mulino 1, Armenia
2 Institute of Molecular Biology of National Academy of Sciences, Hasratyan 7, 0014 Yerevan, Armenia
3 All-Russia Research Institute of Agricultural Biotechnology of RAS, Timiryazevskaya St, 42 Moscow, 117550, Russia
4 N.M. Emanuel Institute of Biochemical Physics, RAS, Kosygina Str.4, Moscow, 119334 Russia
* Corresponding author: gmggmg65@mail.ru
Optimization of in vitro culture conditions of grapevine phylloxera-resistant rootstock cultivar ‘Ruggeri-140’(Vitisberlandieri x Vitisrupestris) was carried out. Among the different sterilization treatments, maximum aseptic cultures were obtained for both explants apical tips and nodal segments when treated with Ca(ClO)2 at concentration of 1.5 % for 10 minutes plus 70 % ethanol for 30 s (T7). The maximum shoot proliferation was observed both in apical and nodal meristems cultured on MS medium supplemented with 1.0 mg/l BAP. MS/2 medium containing 1.0 mg/l indole-3-butric acid (IBA) gave the highest rooting percentage (100%) with the highest mean number and length of roots. The ex vitro survival of rooted micro shoots was 75.0%.
© The Authors, published by EDP Sciences, 2021
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