BIO Web Conf.
Volume 48, 2022The 2nd International Conference “Sport and Healthy Lifestyle Culture in the XXI Century” (SPORT LIFE XXI)
|Number of page(s)||7|
|Section||General Issues of Healthy Food Production|
|Published online||29 June 2022|
Analysis of the probiotic activity of Bacillus velezensis RT-26 strain isolated from reindeer rumen by whole-genome sequencing
OOO BIOTROF+, 19-1 Zagrebsky Blvd., St. Petersburg.
2 St. Petersburg State Agrarian University, 2, Peterburgskoe sh, Saint-Petersburg, Russia
3 St. Petersburg Federal Research Center of the Russian Academy of Sciences, 39, 1-ya liniya V.O., St. Petersburg, Russia
* Corresponding author: ilina@biotrof. ru
The paper analyses the properties of Bacillus velezensis RT-26, a probiotic strain isolated from reindeer rumen, which has high activity towards fiber degradation, against bacterial and fungal pathogens. The analysis was performed using whole-genome sequencing of the strain using the Illumina platform. The study revealed that strain RT-26 possessed a complete set of metabolic pathways, including glycolysis, the tricarboxylic acid cycle, and the pentose phosphate pathway. 411 genes were involved in carbohydrate metabolism in the strain genome, 229 genes were related to vitamin and coenzyme metabolism, 149 genes were involved in fatty acid metabolism. The synthesis pathways of various amino acids, most B vitamins (thiamine, riboflavin, nicotiamide, vitamin B5) were identified in the genome. A complete pathway for synthesis of the dipeptide antibiotic bacilisin was detected in the strain. In addition, the strain is capable of synthesizing class A beta-lactamase. No genes responsible for the degradation of mycotoxins and xenobiotics were detected in the genome of the strain studied. A number of glycosyl hydrolase families were detected in the strain genome: GH 1, 3, 4, 5, 6, 11, 13, 16, 18, 20, 23, 26, 28, 30, 32, 43, 46, 51, 53, 68, 68, 73, 101, 109, 126. Carbohydrate-binding proteins were of the SVM 50 family. Glycosyltransferases were of GT 1, 2, 4, 8, 26, 28, 30, 51, 83 families. In the genome of Bacillus velezensis strain RT-26, cellulases related to families GH 5, 6, 26, 51, chitinases related to families GH 18 and 23, and xylanases related to families GH 1, 3, 4, 16, 30, 43 were found. Thus, strain B. velezensis RT-26 has several phenotypically and genotypically proven properties that can characterize it as a good probiotic microorganism.
© The Authors, published by EDP Sciences, 2022
This is an Open Access article distributed under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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