Effect of chitosan complexes on the bacterial community of cecum and productivity of broiler chickens

¹ Federal Scientific Center “All-Russian Research and Technological Institute of Poultry” of Russian Academy of Sciences, 141311, 10, Ptitsegradskaya Str., Sergiev Posad, Russia
² BIOTROF LLC, 196650, Izhorsky Zavod, bld. 45, Kolpino, St. Petersburg, Russia
³ St. Petersburg State Agrarian University, 196605, 2, Peterburgskoe Sh, St. Petersburg, Russia
⁴ Agrochitin Co., Ltd, 603155, 41, Minina Str., Nizhny Novgorod, Nizhny Novgorod, Russia

^* Corresponding author: egorova_t@vnitip.ru

Abstract

The search and development of natural biological additives that have a comprehensive effect as immunostimulants and improve digestion in poultry is relevant. This study was carried out at the Selection and Genetics Center “Zagorskoe EPH”. The control and experimental groups were formed of the 1-day-old Ross-308 cross broilers (35 heads in each). Six groups were formed. The broilers of the group No.1 (control) received basic feed (BF) with the addition of feed antibiotic Maxus. The group No.2 (control) received BF without feed antibiotic. The broilers of the group No.3 (experimental) received BF and the KH-1 chitosan complex. The group No.4 (experimental) received BF and the KHM chitosan complex with the addition of copper nanoparticles. The group No.5 (experimental) received BF and drinking preparation based on the KH-Aqua chitosan complex. The group No.6 (experimental) received BF and drinking preparation based on the KH-Aqua chitosan complex enriched with copper nanoparticles. The bacterial community of the gut cecum was analyzed using the molecular genetics method of next-generation sequencing (NGS). The addition of chitosan complexes (both supplemented with copper nanoparticles and in the drinking form) made it possible to obtain high livability of broilers with increased live body weight and decreased feed consumption per 1 kg of live body weight gain. The live body weight of 35-day-old broilers in the experimental groups was 2.96-5.70% higher than that of the control with a 5.86-8.23% decrease in feed consumption per 1 kg of live body weight gain. The results of NGS showed that the effect of chitosan complexes on the regulation of the composition of the microbiome of broilers’ cecum was predominantly positive. There was an up to 4.4-fold increase in the content of representatives of the normoflora, bacteria of the family Lactobacillaceae. The number of bacteria of genus Helicobacter, among which pathogens are often found, in the experimental groups was 2.6-33.3 times lower than in the group received antibiotics. So, the chitosan complexes were proved to be valuable supplements for poultry.