BIO Web Conf.
Volume 57, 2023International Scientific and Practical Conference “Innovations, Technological Solutions and Management in Modern Biotechnology and Biomedicine” (ITSM-2022)
|Number of page(s)||9|
|Section||Agrobiotechnology in Crop and Livestock Production|
|Published online||13 January 2023|
Determination of Taxonomic Affiliation and Assessment of Biotechnological Potential of an Indigenous Strain of Gram-Positive Spore-Forming Bacteria
Belgorod State University, Belgorod, Russia
* Corresponding author: firstname.lastname@example.org
The search for new effective strains-producers of enzymes and biologically active substances for the development of new environmentally friendly plant protection products against phytopathogens is a very urgent task for the greening of agriculture. In our work, we used active strains of gram-positive spore-forming bacteria isolated from soil near the roadside zone of the Belgorod region to assess their antagonistic activity to phytopathogens and biotechnological potential. In the course of molecular genetic studies of the 16S rRNA gene of the isolated native strain, a complete similarity of the target with Bacillus subtilis was revealed. When studying morphological and cultural characteristics, it was determined that the cells of the target strain are rod-shaped, arranged singly or in pairs.The conditions of cultivation of the bacterium were revealed, which shows high growth in the composition of the medium containing 3% peptone for 20 hours, the specific increase in biomass exceeded 96%. When evaluating the antagonistic activity, it was revealed that the isolated strain suppresses the growth of cultures of Escherichia coli O157:H7, Aspergillus unguis VKM F-1754 and bacteria of the genus Ralstonia.
© The Authors, published by EDP Sciences, 2023
This is an Open Access article distributed under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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