BIO Web Conf.
Volume 57, 2023International Scientific and Practical Conference “Innovations, Technological Solutions and Management in Modern Biotechnology and Biomedicine” (ITSM-2022)
|Number of page(s)||7|
|Published online||13 January 2023|
Sensitivity of Janthinobacterium Lividum to Low Concentrations of Hydrogen Peroxide and the Effect of Mild Oxidative Stress on Pigment Yield
Belgorod National Research University, Belgorod, Russia
* Corresponding author: email@example.com
In the course of assessing the sensitivity of the Janthinobacterium lividum VKM B-3515 strain to low concentrations of hydrogen peroxide, it was found that at a content of 0.003% H2O2, the growth properties of the bacterium during submerged cultivation without pigmentation differed statistically insignificantly relative to the control variant at 16 hours of incubation and beyond. Whereas in the presence of peroxide at 12 hours the optical density was lower than in the control by 97%. When cultivating by the surface method, the respiration intensity did not significantly differ between the control and experimental variants. However, during the extraction of the pigment, it was found that in the presence of hydrogen peroxide, the optical density of the acetone extract significantly exceeds the control variant by 28%. It can be assumed that, at the same growth parameters of the culture, the biosynthesis of violacein is stimulated and the population can adapt to the peroxide content, and the peroxide concentration itself decreases due to the cost of catalytic reactions. Further studies of the sensitivity of J. lividum VKM B-3515 to various oxidizing agents will allow us to consider the effect of weak oxidative stress on the biosynthesis of violacein.
© The Authors, published by EDP Sciences, 2023
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