| Issue |
BIO Web Conf.
Volume 60, 2023
2022 4th International Conference on Biotechnology and Food Science (BFS 2022)
|
|
|---|---|---|
| Article Number | 01015 | |
| Number of page(s) | 5 | |
| Section | Biochemical Application and Genetic Engineering | |
| DOI | https://doi.org/10.1051/bioconf/20236001015 | |
| Published online | 11 May 2023 | |
Study on Transformation of The Gene of Resveratrol Synthase into Cordyceps militaris
1 Zhuhai College of Science and Technology, Zhuhai, China
2 Beijing University, Beijing, China
* e-mail: 84453594@qq.com
Resveratrol synthase (RS) is a key enzyme in Resveratrol (Res) synthesis pathway. RS genes have been transformed and expressed in many plants and microorganisms, and play biological roles in plant metabolism and regulation. In this study, the full length sequence of resveratrol synthase RS gene was transformed into Cordyceps militaris by Agrobacterium tumefaciens infestation with 1179 bp. The RS gene was integrated into Cordyceps militaris genome by PCR, Southern blot hybridization, Northern hybridization and other molecular biological tests. Objective To improve the content of RS gene in into Cordyceps militaris by transgenic technology, improve the resistance of Cordyceps militaris and promote the content of resveratrol downstream products, provide a theoretical basis for studying the biological function and regulation mechanism of RS gene metabolites, and provide an effective way to improve species, resistance and increase the yield of metabolites.
© The Authors, published by EDP Sciences, 2023
This is an Open Access article distributed under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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