BIO Web Conf.
Volume 67, 2023International Scientific and Practical Conference “VAVILOV READINGS-2023” (VVRD 2023)
|Number of page(s)||10|
|Section||Basic Research in the Field of Plant and Microbial Studies|
|Published online||18 September 2023|
Potential of the rhizobacterium Ensifer meliloti for the phytoremediation of mixed PAH–heavy metal contamination
1 Institute of Biochemistry and Physiology of Plants and Microorganisms, Saratov Scientific Centre of the Russian Academy of Sciences (IBPPM RAS), 410049 Saratov, Russia
2 Chernyshevskii Saratov State University, 410012 Saratov, Russia
* Corresponding author: email@example.com
The potential of the rhizobial strain Ensifer meliloti P221 as a bacterium capable of degrading phenanthrene in the presence of a heavy metal (nickel) and exhibiting plant-growth promoting activity toward Sorghum bicolor under mixed contamination condition was studied. It was shown that nickel at a concentration of 0.1 mM inhibited the microbial growth and phenanthrene degradation by 43 and 34%, respectively. Analysis of the enzymes involved in the phenanthrene degradation by the rhizobium revealed the activity of the phenanthrenequinone reductase (PQR) and protocatechuate 2,3-dioxigenase (2,3-PCD). It was found that nickel at concentrations up to 0.2 mM did not affect significantly the PQR activity, but inhibited it by 30% when the metal concentration increased to 5 mM. Nickel at concentrations up to 5.0 mM did not affect significantly on the activity of 2,3-PCD. E. meliloti promoted elongation of sorghum seedling roots by 69% in medium co-contaminated with phenanthrene and nickel. The results obtained assume that the rhizobial strain studied can exhibit its PAH-degrading and plant-growth promoting activities in the environment co-contaminated with nickel and phenanthrene. This microorganism may be promising for bioremediation and phytoremediation of mixed PAH–heavy metal contamination.
© The Authors, published by EDP Sciences, 2023
This is an Open Access article distributed under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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