Applicable Rapid Propagation Protocol Through Organogenesis of Alocasia Cuprea for Plant Development Using Micro-Bulblet Explant

Research Center for Horticultural and Estate Crops, National Research and Innovation Agency, Jl. Raya Jakarta-Bogor, Cibinong, Bogor, West Java, 16915, Indonesia

^1* Corresponding author: fitri.rachmawati1901@gmail.com

Abstract

Alocasia of Indonesian endemic has significant market potential. However, the scarcity of qualified seedlings hampered their commercialization. The purpose of this study was to obtain the best sterilization technique and media for in vitro propagation of Alocasia cuprea through the organogenesis pathway using microbulblet explants. Randomized complete block designs with five to ten replicates were applied in the experiments. Application of 5 and 10% clorox for 5 and 10 minutes resulted in 40.6% aseptic explants, 10% browning explants, and 90% explant growth potential. The best shoot initiation was achieved on the MS added 4.54 μM thidiazuron and 2.22 μM BAP. This medium had an 85% explant initiation, 4.2 shoots per explant, and 6.5 days initiation time. The best shoot proliferation was discovered on MS medium with 4.44 μM BAP, which produced 10.5 initial shoots with a shoot height of 1.3 cm and a clump diameter of 1.3 cm. The best plantlet regeneration was determined on ½ MS medium with 6.5 cm plantlet height, 3.5 leaves total, 3.8 cm leaf length, 2.6 cm leaf width, and 3.8 roots. This propagation method will be applicable to the propagation of qualified seedlings for commercial purposes and potentially used for other endemic Alocasia.