High Frequencies Repetitive Shoots organogenesis of Potato (Solanum Tuberosum L.) Under Partial Immersion Bioreactor Culture

¹ Badan Riset dan Inovasi Nasional (BRIN), Jalan Raya Surabaya - Malang Km. 65, Sembung Lor, Desa Parerejo, Kecamatan Purwodadi, Kabupaten Pasuruan, Jawa Timur, 67163
² Filant-Corp, Jalan Tarimin 12, Desa Tlekung, Kecamatan Junrejo, Kota Batu, Jawa Timur, 65327

^1* Corresponding author: dita.agisimanto@brin.go.id

Abstract

Potato plays important role in nutritional security, poverty alleviation, and income generation in Indonesia. Potato production restricted and inconsistent by lower quality of seedlings application. Solid medium technique for long time has been using for producing pathogen free plants. This procedure has typically costly as labor intensive and longer durability of production. Liquid culture medium allows the close contact to explants which stimulates and facilitates the nutrients and hormones uptake leading to higher rate multiplication and shoot and root growth of higher quality seedling mother plants. The aim of study is to introduce partial immerse bioreactor system (PIB) for high frequencies of granola Kembang (GK) organogenesis and survival rate during weaning stage in nursery. A 20 shoots of GK were transferred to PIB containing MS medium aerated with 0.5, 1.0 and 1.5 vvm air atmosphere during 30 days of culture. High rate repetitive shoots organogenesis were observed from PIB culture, significantly different to semisolid culture. Averagely 500, 616 and 602 new shoots were harvested from 0.5, 1.0 and 1.5 vvm treatment respectively, which are 30, 37 and 36 folds compared to semisolid culture. Approximately 95-98% of regenerated shoots were survived in acclimatization chamber for both PIB and semisolid culture.