Effect of herbal leaf extract on Oreochromis niloticus immunity infected with Streptococcus agalactiae

¹ Departement of Aquaculture, Marine and Fisheries Faculty, Universitas Syiah Kuala, Banda Aceh, Aceh, 23111 Indonesia
² Research Center for Marine and Fisheries, Universitas Syiah Kuala, Banda Aceh, Aceh, 23111 Indonesia
³ Animal Histology and Fish Nutrition Laboratory, Marine and Fisheries Faculty, Universitas Syiah Kuala, Banda Aceh, Aceh, 23111 Indonesia

^* Corresponding author: ikoimeldaarisa@usk.ac.id

Abstract

Streptococcosis disease caused by the pathogen S. agalactiae is a serious problem in freshwater fish farming. This research aims to use multiherbal ingredients to stimulate the improvement of the fish's immune system in fighting infection by the pathogenic bacteria S. agalactiae. The research was carried out at the FKIP Chemistry Laboratory, the Fish Hatchery and Breeding Laboratory of the FKP, and the Laboratory of the Faculty of Medicine, Syiah Kuala University. The test fish used were tilapia measuring 7-8 cm length. The research method was carried out experimentally using a Completely Randomized Design consisting of 5 treatments and 3 replications, namely treatment A (negative control), B (positive control), C (addition of C. gigantea), D (addition of M. oleivera), E (addition of C. alata L). The test result data is analyzed using Analysis of Variance. The results of research on immersion in multiherbal extracts in fish infected with S. agalactiae showed an increase in the immune response as seen in blood parameters (leukocytes, hemoglobin, hematocrit) and a higher survival rate compared to those without immersion in the extract. The highest survival rate was obtained in the treatment of 10 ppm of C. alata L leaves extract at 83.33%.