Stability Analysis of the Genetic Profile of Drought-Stressed Rice (Oryza sativa L.)

Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang, West Sumatra, 25132, Indonesia

^* Corresponding author: afifatul.achyar@fmipa.unp.ac.id

Abstract

Global climate change might cause a region to have a prolonged dry or rainy season. A long dry season can create drought stress in rice plants, resulting in Reactive Oxygen Species (ROS) compounds in cells and causing damage to plants, one of which is damage to DNA. The purpose of this study is to examine the stability of rice's genetic profile in order to assess the effect of drought stress treatment on rice DNA itself. Previous research classified three rice varieties into three groups based on their drought stress resistance: Harum (tolerant), Situbagendit (moderate), and Rosna (sensitive). These three rice plants were germinated and then treated with drought stress using PEG. Rice roots before and after being treated with drought stress were collected and the DNA was extracted. Genetic profile stability analysis was carried out by RAPD PCR using 10 types of primers. The DNA band patterns in rice samples before and after drought stress were differed, according to the electropherograms of ten RAPD primers and Jaccard's similarity index. This suggests that drought stress may disrupt the genetic stability of the three rice varieties studied.