Evaluation of Antibacterial and Toxicity Properties of Marine Endophytic Fungi from Red Algae Gracilaria Salicornia and Chondrus sp.

Ni Putu Ariantari; I Putu Yogi Astara Putra; J.T. Wibowo; S.A.M.D.A. Putri; Caresya Insani Bangga Nabila; Ni Kadek Ari Kristiani; I Made Agus Gelgel Wirasuta

doi:10.1051/bioconf/20249202019

All issues

Volume 92 (2024)

BIO Web Conf., 92 (2024) 02019

Abstract

Open Access

Issue		BIO Web Conf. Volume 92, 2024 The 4^th International Conference on Integrated Coastal Management & Marine Biotechnology (ICMMBT 2023)


Article Number		02019
Number of page(s)		12
Section		Marine Biotechnology (MBT)
DOI		https://doi.org/10.1051/bioconf/20249202019
Published online		21 February 2024

BIO Web of Conferences 92, 02019 (2024)

Evaluation of Antibacterial and Toxicity Properties of Marine Endophytic Fungi from Red Algae Gracilaria Salicornia and Chondrus sp.

Ni Putu Ariantari¹^*, I Putu Yogi Astara Putra², J.T. Wibowo³, S.A.M.D.A. Putri¹, Caresya Insani Bangga Nabila¹, Ni Kadek Ari Kristiani¹ and I Made Agus Gelgel Wirasuta¹

¹ Department of Pharmacy, Faculty of Mathematics and Natural Sciences, Udayana University, Jl. Raya Kampus Unud, 80361 Badung, Bali, Indonesia
² Department of Biology, Faculty of Mathematics and Natural Sciences, Udayana University, Jl. Raya Kampus Unud, 80361 Badung, Bali, Indonesia
³ Research Center for Vaccine and Drugs, National Research and Innovation Agency (BRIN), Jl. M. H. Tamrin, 10340 Central Jakarta, DKI Jakarta, Indonesia

^* Corresponding author: putu_ariantari@unud.ac.id

Abstract

Marine fungi are widely recognized for producing bioactive compounds with diverse pharmaceutical applications. The study aimed to investigate the antibacterial and cytotoxic properties of methanolic extracts from endophytic fungi derived from Gracilaria salicornia and Chondrus sp., collected from Argani Beach, Bali, Indonesia. Fungal isolation yielded one and three fungal strains from each host, respectively. They were later identified as Aspergillus unguis A4-1-1, Trichoderma yunnanense A5-1-1, Trichoderma asperellum A5-1-2, and Clonostachys pseudostriata A5-2-1 through ITS or LSU region comparison. Each methanolic extract underwent testing for phytochemical content, antibacterial activities, and cytotoxic potentials. Phytochemical screening indicated the presence of alkaloids in all extracts. Notably, the extract of the non-salt-treated fungus A. unguis A4-1-1 demonstrated the most potent activity with MIC values of 15.6 μg/mL against Staphylococcus aureus and Bacillus cereus ATCC 11778, while other extracts showed no substantial activity. Additionally, the salt-treated T. asperellum A5-1-2 exhibited the most potent toxic activity compared to the others with LC₅₀ of 104.9 μg/mL, according to the brine shrimp lethality test. Other extracts showed LC₅₀ values starting from 107.0 to 792.9 μg/mL. Given their notable antibacterial and cytotoxic potentials, it is noteworthy to identify and characterize the bioactive compounds from these fungal strains in future study.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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