| Issue |
BIO Web Conf.
Volume 94, 2024
The 8th International Conference on Biological Sciences “Leveraging Biodiversity to Support Green Economy and Climate Resilience” (ICBS 2023)
|
|
|---|---|---|
| Article Number | 05002 | |
| Number of page(s) | 7 | |
| Section | Genetics, Molecular Biology and Biotechnology | |
| DOI | https://doi.org/10.1051/bioconf/20249405002 | |
| Published online | 25 March 2024 | |
Unveiling Porcine Contamination in Halal-uncertified Cosmetics: Detection through Existing Procedure using DNeasy Mericon Food Kit DNA Extraction and Real-time qPCR
1 Central Lab MMM, Faculty of Mathematics and Natural Sciences, Universitas Negeri Malang
2 Department Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Negeri Malang
3 Health Governance Initiative, United Nations Development Programme Indonesia, Eijkman-RSCM Building, Jakarta, Indonesia
4 Integrated Laboratory Universitas Negeri Malang
* Corresponding author: intan.sartika@um.ac.id, suharti.fmipa@um.ac.id
The surge in cosmetic product consumption in Indonesia has spurred the growth of the local cosmetic industry. However, not all cosmetics in circulation adhere to the halal standards mandated by Indonesian authorities through the Law on Halal Product Guarantee. This study endeavors to establish a method for identifying pork-derived content in cosmetic cream products lacking the halal logo. The approach involves total DNA isolation using the Qiagen DNeasy Mericon Food Kit, followed by detecting the NDH5 gene using qPCR. Results indicated that the isolation kit effectively extracted DNA fragments from cosmetic products, yielding 0.6–0.8 ng/µL concentrations, with A260/280 ratios ranging from 0.90 to 1.76 and A260/230 ratios from 0.12 to 0.17. Analysis of five cosmetic cream samples revealed that three of them exhibited no contamination by pig-derived elements, with a cut-off value of 23.7×101. Consequently, the isolation method utilizing the kit and the subsequent detection of the NDH5 gene through qPCR emerge as a robust approach for identifying pig-derived ingredients in cream-based cosmetics.
© The Authors, published by EDP Sciences, 2024
This is an Open Access article distributed under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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