| Issue |
BIO Web Conf.
Volume 94, 2024
The 8th International Conference on Biological Sciences “Leveraging Biodiversity to Support Green Economy and Climate Resilience” (ICBS 2023)
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|
|---|---|---|
| Article Number | 05001 | |
| Number of page(s) | 11 | |
| Section | Genetics, Molecular Biology and Biotechnology | |
| DOI | https://doi.org/10.1051/bioconf/20249405001 | |
| Published online | 25 March 2024 | |
Polymorphism analysis of the Insulin-like Growth Factor-1 (IGF-1) gene encoding growth in F5 Golden Kamper chicken (Gallus gallus domesticus)
1 Faculty of Biology, Universitas Gadjah Mada, Jl. Teknika Sel., Sendowo, Sinduadi, Kec. Mlati, Kabupaten Sleman, Daerah Istimewa Yogyakarta 55281, Indonesia
* Corresponding author: bs_daryono@mail.ugm.ac.id
Golden Kamper Chicken is a chicken cross between pelung and layer chickens with good meat and egg productivity. Insulin-like growth factor-1 (IGF-1) gene is one factor affecting the speed of chicken growth. This research aims to study the character of F5 Golden Kamper chickens and analyze the IGF-1 gene to determine its effect on chicken growth. The research will be done by hybridizing the F4 Golden Kamper hen and rooster, raising brood stock and DOC, egg collection, growth data collection, blood collection, DNA isolation, DNA amplification, electrophoresis, and sequencing. The observed parameters are DOC weight for the first seven weeks and polymorphism of the IGF-1 gene. Data was analyzed using Microsoft Excel, Genestudio, MEGA11, and IBM SPSS 25 software. Sequencing data were processed using the Genestudio application, alignment with MEGA11, and genotype-haplotype associations using Pearson correlation and Linear Regression F test. The results showed that the molecular marker polymorphism of the IGF-1 gene was found at 12 mutation points. IGF-1 gene polymorphism did not correlate with the weight of F5 Golden Kamper chickens.
© The Authors, published by EDP Sciences, 2024
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